Identification of functional sgRNA mutants lacking canonical secondary structure using high-throughput FACS screening

Zeyu Liang, Chaoyong Huang, Yan Xia, Zhaojin Ye, Shunhua Fan, Junwei Zeng, Shuyuan Guo, Xiaoyan Ma, Lichao Sun, Yi Xin Huo*

*Corresponding author for this work

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Biochemistry, Genetics and Molecular Biology