1. Biocatalytic Engineering
2. Enzyme Engineering
3. Fermentation Engineering
4. Cell Engineering

2003.09-2006.07 Zhejiang University, Major in Biochemistry, Doctor of Engineering
2000.09-2003.07 Zhejiang University, Biochemistry, Master of Engineering
1994.09-1998.07 Huazhong Agricultural University, Biotechnology major, Bachelor of Agriculture

2017.12-present Associate Professor, School of Chemistry and Chemical Engineering, Beijing Institute of Technology
2010.08-2017.12 Associate Professor, School of Life Sciences, Beijing Institute of Technology
2008.08-2010.08 School of Life Sciences, Beijing Institute of Technology, Lecturer
2006.07-2008.07 Postdoc, School of Materials and Chemical Engineering, Zhejiang University

With chiral drugs and their intermediates, bio-based bulk platform compounds and high value-added chemicals as research objects, natural/non-natural enzyme molecular catalysis and recognition mechanism, enzyme molecular structure modification and rational/irrational design, hybrid/evolutionary enzymes and artificial enzyme industrial applications are carried out. And the research work on the construction of cell-free biosynthesis system and cell-like factory by combining catalysis and multi-zymosome technology.
Undertaking Project:
1. Research on the recognition mechanism of phospholipase B interfacial catalysis of Pseudomonas fluorescens and its directional regulation, Supported by the National Natural Science Foundation of China (21276025), 2013.01-2016.12, 780,000 yuan, the first person in charge.
2. Rational Design of hydrazine synthase model protein and its application in Microbial anaerobic ammoxidation, supported by the National Natural Science Foundation of China (21878020), 2019.01-2022.12, 200,000 yuan, Second leader.
3. Molecular mechanism of β-glucuronidase in and out of the substrate catalytic recognition, National Natural Science Foundation of China (21808004), 2019.01-2021.12, 80,000 yuan, second leader.
4. Research on the key technology of clean production of Xylitol by whole enzyme Method, Subproject of the National High Technology Research and Development Program (863 Program) (2009AA02Z202), 2010.01-2013.12, 440,000 yuan, the first person in charge.
5. Basic research on the application of antienantioselective microbial Lipase kinetic dismembering drugs and intermediates, Beijing Institute of Technology Outstanding Young Teachers Funding Program (2009Y1118), 2010.01-2011.12, 40,000 yuan, the first person in charge.
6. Basic research on the application of enantioselective Lipase kinetic Disintegrant drugs and their intermediates, Basic Research Foundation of Beijing Institute of Technology (20081642001), 2009.01-2010.12, 20,000 yuan, First person in charge.
7. Research on Key technologies in Stereoselective Lipase disintegrator drugs, Supported by China Postdoctoral Science Foundation (20060401045), 2007.01-2008.06, 30,000 yuan, First person in charge.
Published article:
So far, he has published more than 50 academic papers in academic journals and conferences at home and abroad, including more than 20 SCI papers, more than 10 EI papers, and 3 authorized patents.
1.Dazhang Dai*, Liming Xia. Enhanced production of Penicillium expansum PED-03 lipase through the control of culture conditions and the application of the crude enzyme in kinetic resolution of racemic allethrolone. Biotech Prog, 2005, 21(4): 1165-1168.
2.Dazhang Dai*, Liming Xia. Resolution of (R, S)-2-octanol by Penicillium expansum PED-03 lipase immobilized on modified ultrastable-Y molecular sieve in microaqueous media. Process Biochem, 2006, 41: 1455~1460.
3.Dazhang Dai*, Liming Xia. Effect of lipase immobilization on the resolution of (R, S)-2-octanol in nonaqueous media using modified ultrastable-Y molecular sieve as support. App Biochem Biotech, 2006, 134(1): 39-50.
4.Dazhang Dai*, Liming Xia. Enzymatic resolution of (R, S)-2-octanol by Ultrastable-Y molecular sieve immobilized-lipase in microaqueous media. Chem J Chin Univ, 2007, 28(12): 2307-2310.
5.Dazhang Dai*, Liming Xia. Production of the lipase from Penicillium expansum PED-03 and its application in the resolution of racemic allethrolone. J Chem Eng Chin Univ, 2008, 22(1): 166-171.
6.Dazhang Dai*, Liming Xia. Effect of immobilization by modified ultrastable-Y molecular sieve on the resolution of (R, S)-2-octanol in nonaqueous media by lipase. Acta chimica sinica, 2008, 66(2): 245-250.
7.Xiaoxiao Guo, Ruihua Zhang, Zhe Li, Dazhang Dai, Chun Li, Xiaohong Zhou. A novel pathway construction in Candida tropicalis for direct xylitol conversion from corncob xylan. Bioresource Technology, 2013, 128: 547~552.
8. Li Wei-xiang, Li Chun, LIU Gui-Yan, Dai Da-Zhang *. Advances in chiral synthesis systems catalyzed by asymmetric enzymes, Molecular Catalysis, 2014, 28(6): 581-594.
9.Guiyan Liu, Dong Wang, Dazhang Dai, Rasool Aamir, Chun Li. Promoting effect of licorice extract on induction of β-glucuronidase in Penicillium purpurogenum Li-3. Journal of Beijing Institute of Technology, 2014,23(1): 138-142.
10.Cuiwei Wang, Zhe Li, Aamir Rasool, Hongna Qu, Dazhang Dai, Chun Li. Characterization of promoters in Escherichia coli and application for xylitol synthesis. Chinese Journal of Chemical Engineering, 2015, 23(1): 234-240.
11. Chen Xinyi; Liu Hu; Dai Dazhang; Li Chun. Study on improving crystallizability of glycosylated enzyme protein, Chinese Journal of Bioengineering, 20,40(03),154-162. 12. Zhao Yi-Wei, Jiang Fang-Yan, Li Chun, Dai Da-Zhang *. Molecular mechanism of phospholipase B interfacial catalysis of Pseudomonas fluorescens, Journal of Chemical Engineering, 20,71(9): 4255-4259.