荧光假单胞杆菌磷脂酶B界面催化分子机制初探

Yiwei Zhao; Fangyan Jiang; Chun Li; Dazhang Dai

doi:10.11949/0438-1157.20200527

荧光假单胞杆菌磷脂酶B界面催化分子机制初探

Translated title of the contribution: Interfacial catalytic mechanism of Pseudomonas fluorescens phospholipase B

Yiwei Zhao, Fangyan Jiang, Chun Li, Dazhang Dai^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A "lid-in, side-out" hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.

Translated title of the contribution	Interfacial catalytic mechanism of Pseudomonas fluorescens phospholipase B
Original language	Chinese (Traditional)
Pages (from-to)	4255-4259
Number of pages	5
Journal	Huagong Xuebao/CIESC Journal
Volume	71
Issue number	9
DOIs	https://doi.org/10.11949/0438-1157.20200527
Publication status	Published - 1 Sept 2020

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title = "荧光假单胞杆菌磷脂酶B界面催化分子机制初探",

abstract = "The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A {"}lid-in, side-out{"} hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.",

keywords = "Catalytic mechanism, Phospholipase B, Structure prediction",

author = "Yiwei Zhao and Fangyan Jiang and Chun Li and Dazhang Dai",

year = "2020",

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doi = "10.11949/0438-1157.20200527",

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T1 - 荧光假单胞杆菌磷脂酶B界面催化分子机制初探

AU - Zhao, Yiwei

AU - Jiang, Fangyan

AU - Li, Chun

AU - Dai, Dazhang

PY - 2020/9/1

Y1 - 2020/9/1

N2 - The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A "lid-in, side-out" hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.

AB - The physicochemical property and secondary structure of Pseudomonas fluorescens phospholipase B(Pf-PLB) were analyzed base on its primary structure, and the tertiary structure of Pf-PLB was predicated. A "lid-in, side-out" hypothesis model was put forward to illuminate the possible interfacial catalytic mechanism of Pf-PLB. The structure of Pf-PLB was modified by site-directed mutagenesis, and the proposed mechanism model was verified by combining with the catalytic properties of mutant Pf-PLB.

KW - Catalytic mechanism

KW - Phospholipase B

KW - Structure prediction

UR - http://www.scopus.com/inward/record.url?scp=85091538939&partnerID=8YFLogxK

U2 - 10.11949/0438-1157.20200527

DO - 10.11949/0438-1157.20200527

M3 - 文章

AN - SCOPUS:85091538939

SN - 0438-1157

VL - 71

SP - 4255

EP - 4259

JO - Huagong Xuebao/CIESC Journal

JF - Huagong Xuebao/CIESC Journal

IS - 9

ER -

荧光假单胞杆菌磷脂酶B界面催化分子机制初探

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