Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

Xiaohong Liu; Yang Yu; Cheng Hu; Wei Zhang; Yi Lu; Jiangyun Wang

doi:10.1002/anie.201108756

Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

Xiaohong Liu^*, Yang Yu, Cheng Hu, Wei Zhang, Yi Lu, Jiangyun Wang

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102 引用（Scopus）

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Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu _B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu _B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O ₂-reduction activity while generating little reactive oxygen species.

源语言	英语
页（从-至）	4312-4316
页数	5
期刊	Angewandte Chemie - International Edition
卷	51
期	18
DOI	https://doi.org/10.1002/anie.201108756
出版状态	已出版 - 27 4月 2012
已对外发布	是

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abstract = "Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.",

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author = "Xiaohong Liu and Yang Yu and Cheng Hu and Wei Zhang and Yi Lu and Jiangyun Wang",

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T1 - Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

AU - Liu, Xiaohong

AU - Yu, Yang

AU - Hu, Cheng

AU - Zhang, Wei

AU - Lu, Yi

AU - Wang, Jiangyun

PY - 2012/4/27

Y1 - 2012/4/27

N2 - Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.

AB - Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.

KW - enzyme catalysis

KW - enzyme models

KW - oxygen reduction

KW - post-translational modifications

KW - protein design

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 18

ER -

Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

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