Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

Xiaohong Liu; Yang Yu; Cheng Hu; Wei Zhang; Yi Lu; Jiangyun Wang

doi:10.1002/anie.201108756

Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

Xiaohong Liu^*, Yang Yu, Cheng Hu, Wei Zhang, Yi Lu, Jiangyun Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

102 Citations (Scopus)

Abstract

Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu _B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu _B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O ₂-reduction activity while generating little reactive oxygen species.

Original language	English
Pages (from-to)	4312-4316
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	18
DOIs	https://doi.org/10.1002/anie.201108756
Publication status	Published - 27 Apr 2012
Externally published	Yes

Keywords

enzyme catalysis
enzyme models
oxygen reduction
post-translational modifications
protein design

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abstract = "Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.",

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AU - Yu, Yang

AU - Hu, Cheng

AU - Zhang, Wei

AU - Lu, Yi

AU - Wang, Jiangyun

PY - 2012/4/27

Y1 - 2012/4/27

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AB - Top model: Heme-copper oxidase (HCO) contains a histidine-tyrosine cross-link in its heme a3/Cu B oxygen reduction center. A functional model of HCO was obtained through the genetic incorporation of the unnatural amino acid imiTyr, which mimics the Tyr-His cross-link, and of the Cu B site into myoglobin (see picture). Like HCO, this small soluble protein exhibits selective O 2-reduction activity while generating little reactive oxygen species.

KW - enzyme catalysis

KW - enzyme models

KW - oxygen reduction

KW - post-translational modifications

KW - protein design

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Significant increase of oxidase activity through the genetic incorporation of a tyrosine-histidine cross-link in a myoglobin model of heme-copper oxidase

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Keywords

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