Mitochondria-specific ultrasensitive ratiometric AIE probe for imaging endogenous peroxynitrite

Bingpeng Guo; Wei Shu; Wenjie Liu; Hong Wang; Shu Xing; Jianbin Chen; Xiaoling Zhang

doi:10.1016/j.snb.2021.130206

Mitochondria-specific ultrasensitive ratiometric AIE probe for imaging endogenous peroxynitrite

Bingpeng Guo^*, Wei Shu, Wenjie Liu, Hong Wang, Shu Xing, Jianbin Chen, Xiaoling Zhang

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Peroxynitrite (ONOO⁻), one of well-known highly reactive oxygen and nitrogen species, is closely related to a quantity of pathological and physiological activities. Studies indicated that mitochondria were the main organelles for the ONOO⁻ production. Therefore, accurately detecting the fluctuations of mitochondrial ONOO⁻ significantly effect in revealing the mechanism of ONOO⁻ in living systems. In this research, a ratiometric fluorescent probe TPE-BOH with AIE characteristic was developed for detecting of ONOO⁻ in living cells. AIEgen TPE-BOH exhibited a quantitative response to ONOO⁻ (50–300 nM) with an ultra-low detection limit (0.45 nM). Remarkably, due to the good biocompatibility and excellent mitochondria-targeted ability of TPE-BOH, it was successfully employed to ratiometric image the fluctuations of ONOO⁻ in mitochondria.

Original language	English
Article number	130206
Journal	Sensors and Actuators, B: Chemical
Volume	344
DOIs	https://doi.org/10.1016/j.snb.2021.130206
Publication status	Published - 1 Oct 2021

Keywords

AIEgen
Mitochondria
ONOO
Ratiometric
Ultrasensitive

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10.1016/j.snb.2021.130206

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title = "Mitochondria-specific ultrasensitive ratiometric AIE probe for imaging endogenous peroxynitrite",

abstract = "Peroxynitrite (ONOO−), one of well-known highly reactive oxygen and nitrogen species, is closely related to a quantity of pathological and physiological activities. Studies indicated that mitochondria were the main organelles for the ONOO− production. Therefore, accurately detecting the fluctuations of mitochondrial ONOO− significantly effect in revealing the mechanism of ONOO− in living systems. In this research, a ratiometric fluorescent probe TPE-BOH with AIE characteristic was developed for detecting of ONOO− in living cells. AIEgen TPE-BOH exhibited a quantitative response to ONOO− (50–300 nM) with an ultra-low detection limit (0.45 nM). Remarkably, due to the good biocompatibility and excellent mitochondria-targeted ability of TPE-BOH, it was successfully employed to ratiometric image the fluctuations of ONOO− in mitochondria.",

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author = "Bingpeng Guo and Wei Shu and Wenjie Liu and Hong Wang and Shu Xing and Jianbin Chen and Xiaoling Zhang",

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AU - Guo, Bingpeng

AU - Shu, Wei

AU - Liu, Wenjie

AU - Wang, Hong

AU - Xing, Shu

AU - Chen, Jianbin

AU - Zhang, Xiaoling

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Peroxynitrite (ONOO−), one of well-known highly reactive oxygen and nitrogen species, is closely related to a quantity of pathological and physiological activities. Studies indicated that mitochondria were the main organelles for the ONOO− production. Therefore, accurately detecting the fluctuations of mitochondrial ONOO− significantly effect in revealing the mechanism of ONOO− in living systems. In this research, a ratiometric fluorescent probe TPE-BOH with AIE characteristic was developed for detecting of ONOO− in living cells. AIEgen TPE-BOH exhibited a quantitative response to ONOO− (50–300 nM) with an ultra-low detection limit (0.45 nM). Remarkably, due to the good biocompatibility and excellent mitochondria-targeted ability of TPE-BOH, it was successfully employed to ratiometric image the fluctuations of ONOO− in mitochondria.

AB - Peroxynitrite (ONOO−), one of well-known highly reactive oxygen and nitrogen species, is closely related to a quantity of pathological and physiological activities. Studies indicated that mitochondria were the main organelles for the ONOO− production. Therefore, accurately detecting the fluctuations of mitochondrial ONOO− significantly effect in revealing the mechanism of ONOO− in living systems. In this research, a ratiometric fluorescent probe TPE-BOH with AIE characteristic was developed for detecting of ONOO− in living cells. AIEgen TPE-BOH exhibited a quantitative response to ONOO− (50–300 nM) with an ultra-low detection limit (0.45 nM). Remarkably, due to the good biocompatibility and excellent mitochondria-targeted ability of TPE-BOH, it was successfully employed to ratiometric image the fluctuations of ONOO− in mitochondria.

KW - AIEgen

KW - Mitochondria

KW - ONOO

KW - Ratiometric

KW - Ultrasensitive

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JO - Sensors and Actuators, B: Chemical

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ER -

Mitochondria-specific ultrasensitive ratiometric AIE probe for imaging endogenous peroxynitrite

Abstract

Keywords

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