In Vitro Light-Up Visualization of a Subunit-Specific Enzyme by an AIE Probe via Restriction of Single Molecular Motion

Tienan Zang, Yachen Xie, Sa Su, Feiran Liu, Qianqian Chen, Jing Jing, Rubo Zhang*, Guangle Niu, Xiaoling Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Enzymes contain several subunits to maintain different biological functions. However, it remains a great challenge for specific discrimination of one subunit over another. Toward this end, the fluorescent probe TPEMA is now presented for highly specific detection of the B subunit of cytosolic creatine (CK) kinase isoenzyme (CK-B). Owing to its aggregation-induced emission property, TPEMA shows highly boosted emission toward CK-B with a fast response time and very low interference from other analytes, including the M subunit of CK (CK-M). With the aid of a Job plot assay, ITC assay and molecular dynamics simulation, it was directly confirmed that the remarkably enhanced fluorescence of TPEMA in the presence of CK-B results from the restriction of single molecular motion in the cavity. Selective wash-free fluorescence imaging of CK-B in macrophages under different treatments was successfully demonstrated.

Original languageEnglish
Pages (from-to)10003-10007
Number of pages5
JournalAngewandte Chemie - International Edition
Volume59
Issue number25
DOIs
Publication statusPublished - 15 Jun 2020

Keywords

  • aggregation-induced emission
  • creatine kinase isoenzyme
  • fluorescence imaging
  • fluorescent probes

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Zang, T., Xie, Y., Su, S., Liu, F., Chen, Q., Jing, J., Zhang, R., Niu, G., & Zhang, X. (2020). In Vitro Light-Up Visualization of a Subunit-Specific Enzyme by an AIE Probe via Restriction of Single Molecular Motion. Angewandte Chemie - International Edition, 59(25), 10003-10007. https://doi.org/10.1002/anie.201915783