Improving the thermo-tolerance of yeast base on the antioxidant defense system

Ke Xu; Liman Gao; Jalees Ul Hassan; Zhiping Zhao; Chun Li; Yi Xin Huo; Guiyan Liu

doi:10.1016/j.ces.2017.10.016

Improving the thermo-tolerance of yeast base on the antioxidant defense system

Ke Xu, Liman Gao, Jalees Ul Hassan, Zhiping Zhao, Chun Li, Yi Xin Huo, Guiyan Liu^*

^*Corresponding author for this work

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Heat stress in yeast limits productivity in the industrial production of bio-ethanol. One promising way for enhancing yeast resistance to heat is to strengthen the antioxidant defense system. Herein, we developed an artificial antioxidant defense system and used it to improve the thermo-tolerance of yeast for the first time. Engineered strains not only exhibited high-performance on tolerance to high temperature but also effectively improved the ethanol yield. A-SOD2-TTHA1300 showed the highest ethanol yield, 61.4 g/L at 60 h, which is increased by 66% compared with the control. Furthermore, we explored the damage mechanism of cells under heat stress and oxidative stress by detection of mitochondria and cell membrane integrity. These results together demonstrated the great potential of artificial antioxidant defense system in improving the thermo-tolerance in yeast.

Original language	English
Pages (from-to)	335-342
Number of pages	8
Journal	Chemical Engineering Science
Volume	175
DOIs	https://doi.org/10.1016/j.ces.2017.10.016
Publication status	Published - 16 Jan 2018

Keywords

Antioxidant defense system
Ethanol
Oxidative stress
Thermo-tolerance
Yeast

Access to Document

10.1016/j.ces.2017.10.016

Cite this

@article{881ba8bdd1544c58b668460613d43e9c,

title = "Improving the thermo-tolerance of yeast base on the antioxidant defense system",

abstract = "Heat stress in yeast limits productivity in the industrial production of bio-ethanol. One promising way for enhancing yeast resistance to heat is to strengthen the antioxidant defense system. Herein, we developed an artificial antioxidant defense system and used it to improve the thermo-tolerance of yeast for the first time. Engineered strains not only exhibited high-performance on tolerance to high temperature but also effectively improved the ethanol yield. A-SOD2-TTHA1300 showed the highest ethanol yield, 61.4 g/L at 60 h, which is increased by 66% compared with the control. Furthermore, we explored the damage mechanism of cells under heat stress and oxidative stress by detection of mitochondria and cell membrane integrity. These results together demonstrated the great potential of artificial antioxidant defense system in improving the thermo-tolerance in yeast.",

keywords = "Antioxidant defense system, Ethanol, Oxidative stress, Thermo-tolerance, Yeast",

author = "Ke Xu and Liman Gao and Hassan, {Jalees Ul} and Zhiping Zhao and Chun Li and Huo, {Yi Xin} and Guiyan Liu",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2018",

month = jan,

day = "16",

doi = "10.1016/j.ces.2017.10.016",

language = "English",

volume = "175",

pages = "335--342",

journal = "Chemical Engineering Science",

issn = "0009-2509",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Improving the thermo-tolerance of yeast base on the antioxidant defense system

AU - Xu, Ke

AU - Gao, Liman

AU - Hassan, Jalees Ul

AU - Zhao, Zhiping

AU - Li, Chun

AU - Huo, Yi Xin

AU - Liu, Guiyan

PY - 2018/1/16

Y1 - 2018/1/16

N2 - Heat stress in yeast limits productivity in the industrial production of bio-ethanol. One promising way for enhancing yeast resistance to heat is to strengthen the antioxidant defense system. Herein, we developed an artificial antioxidant defense system and used it to improve the thermo-tolerance of yeast for the first time. Engineered strains not only exhibited high-performance on tolerance to high temperature but also effectively improved the ethanol yield. A-SOD2-TTHA1300 showed the highest ethanol yield, 61.4 g/L at 60 h, which is increased by 66% compared with the control. Furthermore, we explored the damage mechanism of cells under heat stress and oxidative stress by detection of mitochondria and cell membrane integrity. These results together demonstrated the great potential of artificial antioxidant defense system in improving the thermo-tolerance in yeast.

AB - Heat stress in yeast limits productivity in the industrial production of bio-ethanol. One promising way for enhancing yeast resistance to heat is to strengthen the antioxidant defense system. Herein, we developed an artificial antioxidant defense system and used it to improve the thermo-tolerance of yeast for the first time. Engineered strains not only exhibited high-performance on tolerance to high temperature but also effectively improved the ethanol yield. A-SOD2-TTHA1300 showed the highest ethanol yield, 61.4 g/L at 60 h, which is increased by 66% compared with the control. Furthermore, we explored the damage mechanism of cells under heat stress and oxidative stress by detection of mitochondria and cell membrane integrity. These results together demonstrated the great potential of artificial antioxidant defense system in improving the thermo-tolerance in yeast.

KW - Antioxidant defense system

KW - Ethanol

KW - Oxidative stress

KW - Thermo-tolerance

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=85031718679&partnerID=8YFLogxK

U2 - 10.1016/j.ces.2017.10.016

DO - 10.1016/j.ces.2017.10.016

M3 - Article

AN - SCOPUS:85031718679

SN - 0009-2509

VL - 175

SP - 335

EP - 342

JO - Chemical Engineering Science

JF - Chemical Engineering Science

ER -

Improving the thermo-tolerance of yeast base on the antioxidant defense system

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this