Abstract
Pseudomonas fluorescens BIT-18 was isolated from soil near a vegetable oil factory and shown to produce a B-type phospholipase. The enzyme was partially purified by ammonium sulfate precipitation. Gas chromatography demonstrated that the enzyme preparation hydrolyzed both the 1- and 2-ester bonds of phosphatidylcholine. When degumming of soybean, rapeseed, and peanut oil was performed with this enzyme preparation, oils with phosphorous contents lower than 5. mg/kg were obtained after 5. h of enzyme treatment at 40°C. The enzyme preparation did not show lipase activity, thus free fatty acids were only generated from the phospholipids. Therefore, this novel phospholipase B is potentially useful for the refining of high-quality oils with attractive yields.
Original language | English |
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Pages (from-to) | 8052-8056 |
Number of pages | 5 |
Journal | Bioresource Technology |
Volume | 102 |
Issue number | 17 |
DOIs | |
Publication status | Published - Sept 2011 |
Keywords
- Degumming
- Phospholipase B
- Pseudomonas fluorescens
- Vegetable oils