Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18

Fangyan Jiang, Jinmei Wang, Imdad Kaleem, Dazhang Dai, Xiaohong Zhou*, Chun Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Pseudomonas fluorescens BIT-18 was isolated from soil near a vegetable oil factory and shown to produce a B-type phospholipase. The enzyme was partially purified by ammonium sulfate precipitation. Gas chromatography demonstrated that the enzyme preparation hydrolyzed both the 1- and 2-ester bonds of phosphatidylcholine. When degumming of soybean, rapeseed, and peanut oil was performed with this enzyme preparation, oils with phosphorous contents lower than 5. mg/kg were obtained after 5. h of enzyme treatment at 40°C. The enzyme preparation did not show lipase activity, thus free fatty acids were only generated from the phospholipids. Therefore, this novel phospholipase B is potentially useful for the refining of high-quality oils with attractive yields.

Original languageEnglish
Pages (from-to)8052-8056
Number of pages5
JournalBioresource Technology
Volume102
Issue number17
DOIs
Publication statusPublished - Sept 2011

Keywords

  • Degumming
  • Phospholipase B
  • Pseudomonas fluorescens
  • Vegetable oils

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Jiang, F., Wang, J., Kaleem, I., Dai, D., Zhou, X., & Li, C. (2011). Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18. Bioresource Technology, 102(17), 8052-8056. https://doi.org/10.1016/j.biortech.2011.05.050