Cellulosimicrobium composti sp. Nov., a thermophilic bacterium isolated from compost

A Gram-stain-positive, yellow-pigmented, non-motile actinobacterial strain, designated as BIT-GX5^T, was isolated from a sesame husks compost collected in Beijing, PR China. This bacterium was found to be able to grow in the temperature range from 16 to 50 °C and had an optimal growth temperature at 45 °C. Its taxonomic position was analysed using a polyphasic approach. The 16S rRNA gene sequence (1482 bp) of strain BIT-GX5^T was most similar to Cellulosimicrobium funkei ATCC BAA-886^T (99.45%), Cellulo-simicrobium cellulans LMG 16121^T (99.17%) and Cellulosimicrobium marinum RS-7-4^T (98.75%). The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of five housekeeping genes (gyrB, rpoB, recA, atpD and trpB) and genome sequences, placed strain BIT-GX5^T in a separate lineage among the genus Cellulosimicrobium within the family Promicromono-sporaceae. The major polar lipids of strain BIT-GX5^T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid and aminolipid. The major isoprenoid quinone was MK-9(H₄ ), while the cell-wall sugars were galactose, rham-nose, glucose and mannose. The peptidoglycan type was A4α l-Lys–d-Ser–d-Asp. The major fatty acids were anteiso-C_15:0 and iso-C_15:0, which were similar to other members in the genus Cellulosimicrobium. Results of in silico DNA–DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests exhibited the genotypic and phenotypic differentiation of strain BIT-GX5^T from the other members of the genus Cellulosimicrobium. Therefore, strain BIT-GX5^T is considered to represent a novel species within the genus Cellulosimicrobium, for which the name Cellulosimicrobium composti sp. nov. is proposed. The type strain is BIT-GX5^T (=CGMCC 1.17687^T=KCTC 49391^T).