T₁-Mediated Nanosensor for Immunoassay Based on an Activatable MnO₂ Nanoassembly

Current magnetic relaxation switching (MRS) sensors for detection of trace targets in complex samples still suffer from limitations in terms of relatively low sensitivity and poor stability. To meet this challenge, we develop a longitudinal relaxation time (T₁)-based nanosensor by using Mn²⁺ released from the reduction of a MnO₂ nanoassembly that can induce the change of T₁, and thus can greatly improve the sensitivity and overcome the "hook effect" of conventional MRS. Through the specific interaction between antigen and the antibody-functionalized MnO₂ nanoassembly, the T₁ signal of Mn²⁺ released from the nanoassembly is quantitatively determined by the antigen, which allows for highly sensitive and straightforward detection of targets. This approach broadens the applicability of magnetic biosensors and has great potential for applications in early diagnosis of disease biomarkers.