TY - GEN
T1 - A Microfluidic chip for monoclonal antibody functional screening
AU - Xi, Jiyu
AU - Wei, Zewen
AU - Zhang, Anlan
AU - Ma, Tianjiao
N1 - Publisher Copyright:
© 2024 Copyright held by the owner/author(s).
PY - 2024/1/12
Y1 - 2024/1/12
N2 - Monoclonal antibodies, secreted by a single clone of B cells, possess excellent specificity and high sensitivity. Due to their outstanding advantages, the monoclonal antibody market is overgrowing. Currently, the preparation of monoclonal antibodies mainly relies on hybridoma technology, which preserves the biological activity and increases the antibody yield. However, the time-consuming cell screening and low throughput in hybridoma technology greatly limit the antibody screening process. In this study, a microfluidic chip for antibody functional screening was developed by combining hybridoma technology with single-cell technology. The microarraybased approach significantly improved the screening throughput and reduced the screening time.
AB - Monoclonal antibodies, secreted by a single clone of B cells, possess excellent specificity and high sensitivity. Due to their outstanding advantages, the monoclonal antibody market is overgrowing. Currently, the preparation of monoclonal antibodies mainly relies on hybridoma technology, which preserves the biological activity and increases the antibody yield. However, the time-consuming cell screening and low throughput in hybridoma technology greatly limit the antibody screening process. In this study, a microfluidic chip for antibody functional screening was developed by combining hybridoma technology with single-cell technology. The microarraybased approach significantly improved the screening throughput and reduced the screening time.
KW - antibody screening
KW - hybridoma technology
KW - microfluidic chip
UR - http://www.scopus.com/inward/record.url?scp=85187791221&partnerID=8YFLogxK
U2 - 10.1145/3640900.3640905
DO - 10.1145/3640900.3640905
M3 - Conference contribution
AN - SCOPUS:85187791221
T3 - ACM International Conference Proceeding Series
SP - 60
EP - 64
BT - ICBBB 2024 - 2024 14th International Conference on Bioscience, Biochemistry and Bioinformatics
PB - Association for Computing Machinery
T2 - 14th International Conference on Bioscience, Biochemistry and Bioinformatics, ICBBB 2024
Y2 - 12 January 2024 through 15 January 2024
ER -