TY - JOUR
T1 - Immobilization of carbonic anhydrase on polyvinylidene fluoride membranes
AU - Sun, Jing
AU - Wei, Lina
AU - Wang, Yanzi
AU - Zhao, Zhiping
AU - Liu, Wenfang
N1 - Publisher Copyright:
© 2017 International Union of Biochemistry and Molecular Biology, Inc.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - In recent years, the application of carbonic anhydrase (CA) in CO2 removal has attracted great interest. However, obtaining high enzyme recovery activity is difficult in existing immobilization techniques. In this work, water plasma–treated poly(vinylidene fluoride) (PVDF) membranes were modified via 3-aminopropyl triethoxy silane (KH550) or γ-(2, 3-epoxypropoxy) propyl trimethoxy silane (KH560), and then CA was attached. The immobilization process was optimized, and the catalytic properties of PVDF-attached CA were characterized. The maximum activity recovery of PVDF-KH550-CA was 60%, whereas that of PVDF-KH560-CA was 33%. The Km values of PVDF-KH550-CA, PVDF-KH560-CA, and free enzyme were 9.97 ± 0.37, 12.5 ± 0.2, and 6.18 ± 0.23 mM, respectively, and their Kcat/Km values were 206 ± 2, 117 ± 5, and 488 ± 4 M−1·Sec−1. PVDF-attached CA shows excellent storage stability and reusability, and their half-life values were 82 and 78 days at 4 °C. At 25 °C, they were 50 and 37 days, respectively. PVDF-KH550-CA and PVDF-KH560-CA retained approximately 85% and 72% of the initial activity after undergoing 10 cycles. In the presence of them, the generation rates of CaCO3 were 76% and 65% of the free CA system, which were 1.6 and 1.3 times that of the blank system, respectively. Its role in accelerating CO2 sequestration holds great promise for its practical application.
AB - In recent years, the application of carbonic anhydrase (CA) in CO2 removal has attracted great interest. However, obtaining high enzyme recovery activity is difficult in existing immobilization techniques. In this work, water plasma–treated poly(vinylidene fluoride) (PVDF) membranes were modified via 3-aminopropyl triethoxy silane (KH550) or γ-(2, 3-epoxypropoxy) propyl trimethoxy silane (KH560), and then CA was attached. The immobilization process was optimized, and the catalytic properties of PVDF-attached CA were characterized. The maximum activity recovery of PVDF-KH550-CA was 60%, whereas that of PVDF-KH560-CA was 33%. The Km values of PVDF-KH550-CA, PVDF-KH560-CA, and free enzyme were 9.97 ± 0.37, 12.5 ± 0.2, and 6.18 ± 0.23 mM, respectively, and their Kcat/Km values were 206 ± 2, 117 ± 5, and 488 ± 4 M−1·Sec−1. PVDF-attached CA shows excellent storage stability and reusability, and their half-life values were 82 and 78 days at 4 °C. At 25 °C, they were 50 and 37 days, respectively. PVDF-KH550-CA and PVDF-KH560-CA retained approximately 85% and 72% of the initial activity after undergoing 10 cycles. In the presence of them, the generation rates of CaCO3 were 76% and 65% of the free CA system, which were 1.6 and 1.3 times that of the blank system, respectively. Its role in accelerating CO2 sequestration holds great promise for its practical application.
KW - CO absorption
KW - carbonic anhydrase, immobilization, poly(vinylidene fluoride), silane-coupling agent
UR - http://www.scopus.com/inward/record.url?scp=85040056797&partnerID=8YFLogxK
U2 - 10.1002/bab.1629
DO - 10.1002/bab.1629
M3 - Article
C2 - 29222863
AN - SCOPUS:85040056797
SN - 0885-4513
VL - 65
SP - 362
EP - 371
JO - Biotechnology and Applied Biochemistry
JF - Biotechnology and Applied Biochemistry
IS - 3
ER -