A high-resolution interaction map of three transcriptional activation domains with a key coactivator from photo-cross-linking and multiplexed mass spectrometry

Chinmay Y. Majmudar; Bo Wang; Jenifer K. Lum; Kristina Hâkansson; Anna K. Mapp

doi:10.1002/anie.200902669

A high-resolution interaction map of three transcriptional activation domains with a key coactivator from photo-cross-linking and multiplexed mass spectrometry

Chinmay Y. Majmudar, Bo Wang, Jenifer K. Lum, Kristina Hâkansson, Anna K. Mapp

University of Michigan, Ann Arbor

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Binding with distinction: Photo-cross-linking followed by the complementary use of ESI and MALDI mass spectrometric techniques yielded the interaction sites of three prototypical amphipathic transcriptional activators (represented by different-colored helices in the picture) with the coactivator Med15. Investigation of the functional relevance of these sites in yeast revealed overlapping yet distinct binding patterns for the activators investigated.

Original language	English
Pages (from-to)	7021-7024
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	48
Issue number	38
DOIs	https://doi.org/10.1002/anie.200902669
Publication status	Published - 7 Sept 2009
Externally published	Yes

Keywords

Coactivator binding sites
Cross-linking
Mass spectrometry
Photochemistry
Transcriptional activators

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10.1002/anie.200902669

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abstract = "Binding with distinction: Photo-cross-linking followed by the complementary use of ESI and MALDI mass spectrometric techniques yielded the interaction sites of three prototypical amphipathic transcriptional activators (represented by different-colored helices in the picture) with the coactivator Med15. Investigation of the functional relevance of these sites in yeast revealed overlapping yet distinct binding patterns for the activators investigated.",

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A high-resolution interaction map of three transcriptional activation domains with a key coactivator from photo-cross-linking and multiplexed mass spectrometry. / Majmudar, Chinmay Y.; Wang, Bo; Lum, Jenifer K. et al.
In: Angewandte Chemie - International Edition, Vol. 48, No. 38, 07.09.2009, p. 7021-7024.

Research output: Contribution to journal › Article › peer-review

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AU - Hâkansson, Kristina

AU - Mapp, Anna K.

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A high-resolution interaction map of three transcriptional activation domains with a key coactivator from photo-cross-linking and multiplexed mass spectrometry

Abstract

Keywords

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