Optimization of oligonucleotide pools amplification for SELEX

Cong Xiao Zhang, Xue Fei Lv, Xiang Han Wang, Hong Qing, Yu Lin Deng*

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

摘要

In order to realize the amplification of oligonucleotide pools for aptamer selection, this investigation studied the Optimization of oligonucleotide amplification pools for SELEX. The PCR conditions were optimized including cycles, annealing temperature, primer concentrations and template concentrations. The study will be helpful with the aptamer selection for aptamer candidate amplification.

源语言英语
主期刊名Medicine Sciences and Bioengineering - Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
编辑Mings Wang
出版商CRC Press/Balkema
749-752
页数4
ISBN(电子版)9781138026841
DOI
出版状态已出版 - 2015
活动Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014 - Kunming, 中国
期限: 16 8月 201417 8月 2014

出版系列

姓名Medicine Sciences and Bioengineering - Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014

会议

会议Proceedings of the 2014 International Conference on Medicine Sciences and Bioengineering, ICMSB 2014
国家/地区中国
Kunming
时期16/08/1417/08/14

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