Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain

Xuechai Chen, Abida Arshad, Xiaoyin Lin, Yi Chen, Jing Shen, Yongqian Zhang, Yulin Deng*

*此作品的通讯作者

科研成果: 书/报告/会议事项章节章节同行评审

摘要

Several studies suggest that l-methyl-6, 7-dihydroxy-l, 2, 3, 4 - tetrahydroisoquinoline (Salsolinol, Sal) is toxic to dopaminergic neurons, and it is one of the most discussed tetrahydroisoquinolines whose chemical structure is the same with MPTP, it may cause PD. Studies show that salsolinol is biosynthesized by a novel enzyme named salsolinol synthase in human brain. Salsolinol synthase is one kind of enzyme catalyzes the condensation of dopamine and acetaldehyde. It was preliminary purified from rat brains first time in this paper. The purification procedure involved ultracentrifugation, DEAE anion exchange chromatography and gel filtration chromatography. HPLC-ECD was used for enzyme activity detection during purification. Salsolinol synthase was purified 2753.1 fold from the crude enzyme extract.

源语言英语
主期刊名5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
出版商IEEE Computer Society
ISBN(印刷版)9781424450893
DOI
出版状态已出版 - 2011

出版系列

姓名5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

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