Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain

Xuechai Chen; Abida Arshad; Xiaoyin Lin; Yi Chen; Jing Shen; Yongqian Zhang; Yulin Deng

doi:10.1109/icbbe.2011.5780133

Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain

Xuechai Chen, Abida Arshad, Xiaoyin Lin, Yi Chen, Jing Shen, Yongqian Zhang, Yulin Deng^*

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Several studies suggest that l-methyl-6, 7-dihydroxy-l, 2, 3, 4 - tetrahydroisoquinoline (Salsolinol, Sal) is toxic to dopaminergic neurons, and it is one of the most discussed tetrahydroisoquinolines whose chemical structure is the same with MPTP, it may cause PD. Studies show that salsolinol is biosynthesized by a novel enzyme named salsolinol synthase in human brain. Salsolinol synthase is one kind of enzyme catalyzes the condensation of dopamine and acetaldehyde. It was preliminary purified from rat brains first time in this paper. The purification procedure involved ultracentrifugation, DEAE anion exchange chromatography and gel filtration chromatography. HPLC-ECD was used for enzyme activity detection during purification. Salsolinol synthase was purified 2753.1 fold from the crude enzyme extract.

Original language	English
Title of host publication	5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
Publisher	IEEE Computer Society
ISBN (Print)	9781424450893
DOIs	https://doi.org/10.1109/icbbe.2011.5780133
Publication status	Published - 2011

Publication series

Name	5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

Keywords

Gel filtration
Ion-exchange
Purification
Salsolinol synthase

Access to Document

10.1109/icbbe.2011.5780133

Cite this

Chen, X., Arshad, A., Lin, X., Chen, Y., Shen, J., Zhang, Y., & Deng, Y. (2011). Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain. In 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 Article 5780133 (5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011). IEEE Computer Society. https://doi.org/10.1109/icbbe.2011.5780133

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title = "Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain",

abstract = "Several studies suggest that l-methyl-6, 7-dihydroxy-l, 2, 3, 4 - tetrahydroisoquinoline (Salsolinol, Sal) is toxic to dopaminergic neurons, and it is one of the most discussed tetrahydroisoquinolines whose chemical structure is the same with MPTP, it may cause PD. Studies show that salsolinol is biosynthesized by a novel enzyme named salsolinol synthase in human brain. Salsolinol synthase is one kind of enzyme catalyzes the condensation of dopamine and acetaldehyde. It was preliminary purified from rat brains first time in this paper. The purification procedure involved ultracentrifugation, DEAE anion exchange chromatography and gel filtration chromatography. HPLC-ECD was used for enzyme activity detection during purification. Salsolinol synthase was purified 2753.1 fold from the crude enzyme extract.",

keywords = "Gel filtration, Ion-exchange, Purification, Salsolinol synthase",

author = "Xuechai Chen and Abida Arshad and Xiaoyin Lin and Yi Chen and Jing Shen and Yongqian Zhang and Yulin Deng",

year = "2011",

doi = "10.1109/icbbe.2011.5780133",

language = "English",

isbn = "9781424450893",

series = "5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011",

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address = "United States",

}

Chen, X, Arshad, A, Lin, X, Chen, Y, Shen, J, Zhang, Y & Deng, Y 2011, Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain. in 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011., 5780133, 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011, IEEE Computer Society. https://doi.org/10.1109/icbbe.2011.5780133

Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain. / Chen, Xuechai; Arshad, Abida; Lin, Xiaoyin et al.
5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. IEEE Computer Society, 2011. 5780133 (5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Notice of Retraction

T2 - Preliminary purification of salsolinol synthase from rat brain

AU - Chen, Xuechai

AU - Arshad, Abida

AU - Lin, Xiaoyin

AU - Chen, Yi

AU - Shen, Jing

AU - Zhang, Yongqian

AU - Deng, Yulin

PY - 2011

Y1 - 2011

N2 - Several studies suggest that l-methyl-6, 7-dihydroxy-l, 2, 3, 4 - tetrahydroisoquinoline (Salsolinol, Sal) is toxic to dopaminergic neurons, and it is one of the most discussed tetrahydroisoquinolines whose chemical structure is the same with MPTP, it may cause PD. Studies show that salsolinol is biosynthesized by a novel enzyme named salsolinol synthase in human brain. Salsolinol synthase is one kind of enzyme catalyzes the condensation of dopamine and acetaldehyde. It was preliminary purified from rat brains first time in this paper. The purification procedure involved ultracentrifugation, DEAE anion exchange chromatography and gel filtration chromatography. HPLC-ECD was used for enzyme activity detection during purification. Salsolinol synthase was purified 2753.1 fold from the crude enzyme extract.

AB - Several studies suggest that l-methyl-6, 7-dihydroxy-l, 2, 3, 4 - tetrahydroisoquinoline (Salsolinol, Sal) is toxic to dopaminergic neurons, and it is one of the most discussed tetrahydroisoquinolines whose chemical structure is the same with MPTP, it may cause PD. Studies show that salsolinol is biosynthesized by a novel enzyme named salsolinol synthase in human brain. Salsolinol synthase is one kind of enzyme catalyzes the condensation of dopamine and acetaldehyde. It was preliminary purified from rat brains first time in this paper. The purification procedure involved ultracentrifugation, DEAE anion exchange chromatography and gel filtration chromatography. HPLC-ECD was used for enzyme activity detection during purification. Salsolinol synthase was purified 2753.1 fold from the crude enzyme extract.

KW - Gel filtration

KW - Ion-exchange

KW - Purification

KW - Salsolinol synthase

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DO - 10.1109/icbbe.2011.5780133

M3 - Chapter

AN - SCOPUS:79960115425

SN - 9781424450893

T3 - 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

BT - 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

PB - IEEE Computer Society

ER -

Chen X, Arshad A, Lin X, Chen Y, Shen J, Zhang Y et al. Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain. In 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. IEEE Computer Society. 2011. 5780133. (5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011). doi: 10.1109/icbbe.2011.5780133

Notice of Retraction: Preliminary purification of salsolinol synthase from rat brain

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