Capillary electrophoretic enantiomeric separations with 2,6-di -O-carboxymethyl -β-cyclodextrin

The water soluble 2,6-di-O-carboxymethyl-β-cyclodextrin(CM-β-CD) was used as a chiral selector to separate chloramphenicol metabolite. The effects of pH and CM-β-CD concentration on the enantiomeric separation were investigated. The addition of an organic modifier (methanol) to the buffer led to deterioration of the enantiomeric separation. The results indicated that CM-β-CD was superior to β-CD for the separation of chloramphenicol metabolite and the interaction mechanisms of CM-β-CD and β-CD were compared.