TY - JOUR
T1 - Advance and Application of Single-cell Transcriptomics in Auditory Research
AU - Ma, Xiangyu
AU - Guo, Jiamin
AU - Tian, Mengyao
AU - Fu, Yaoyang
AU - Jiang, Pei
AU - Zhang, Yuan
AU - Chai, Renjie
N1 - Publisher Copyright:
© Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences 2023.
PY - 2024/7
Y1 - 2024/7
N2 - Hearing loss and deafness, as a worldwide disability disease, have been troubling human beings. However, the auditory organ of the inner ear is highly heterogeneous and has a very limited number of cells, which are largely uncharacterized in depth. Recently, with the development and utilization of single-cell RNA sequencing (scRNA-seq), researchers have been able to unveil the complex and sophisticated biological mechanisms of various types of cells in the auditory organ at the single-cell level and address the challenges of cellular heterogeneity that are not resolved through by conventional bulk RNA sequencing (bulk RNA-seq). Herein, we reviewed the application of scRNA-seq technology in auditory research, with the aim of providing a reference for the development of auditory organs, the pathogenesis of hearing loss, and regenerative therapy. Prospects about spatial transcriptomic scRNA-seq, single-cell based genome, and Live-seq technology will also be discussed.
AB - Hearing loss and deafness, as a worldwide disability disease, have been troubling human beings. However, the auditory organ of the inner ear is highly heterogeneous and has a very limited number of cells, which are largely uncharacterized in depth. Recently, with the development and utilization of single-cell RNA sequencing (scRNA-seq), researchers have been able to unveil the complex and sophisticated biological mechanisms of various types of cells in the auditory organ at the single-cell level and address the challenges of cellular heterogeneity that are not resolved through by conventional bulk RNA sequencing (bulk RNA-seq). Herein, we reviewed the application of scRNA-seq technology in auditory research, with the aim of providing a reference for the development of auditory organs, the pathogenesis of hearing loss, and regenerative therapy. Prospects about spatial transcriptomic scRNA-seq, single-cell based genome, and Live-seq technology will also be discussed.
KW - Auditory sensory epithelium
KW - Cochlear
KW - Inner ear
KW - Single-cell RNA sequencing
KW - Spiral ganglion neuron
UR - http://www.scopus.com/inward/record.url?scp=85177859389&partnerID=8YFLogxK
U2 - 10.1007/s12264-023-01149-z
DO - 10.1007/s12264-023-01149-z
M3 - Review article
AN - SCOPUS:85177859389
SN - 1673-7067
VL - 40
SP - 963
EP - 980
JO - Neuroscience Bulletin
JF - Neuroscience Bulletin
IS - 7
ER -