A novel real-time TMAO detection method based on microbial electrochemical technology

Yue Yi, Axin Liang, Lin Luo, Yuxuan Zang, Hongyu Zhao, Aiqin Luo*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

15 引用 (Scopus)

摘要

Trimethylamine N-oxide (TMAO) is considered to be a novel biomarker of cardiovascular diseases. However, the traditional TMAO detection method has failed to meet the requirements of real-time and point-of-care tests. Herein, a novel TMAO detection method based on microbial electrochemical technology is established, which realizes the direct conversion of TMAO concentration into electrical signals. Attached Shewanella loihica PV-4 was first proven to be capable of simultaneous inward extracellular electron transfer and TMAO reduction. The TMAO detection method showed a wide linear range of 0 to 250 μM, a high sensitivity of 23.92 μA/mM, and a low limit of detection of 5.96 μM. In addition, the TMAO detection process was accomplished within 600 s, with an acceptable accuracy of 90% in the real serum, showing high feasibility in clinical applications.

源语言英语
文章编号108038
期刊Bioelectrochemistry
144
DOI
出版状态已出版 - 4月 2022

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