A novel real-time TMAO detection method based on microbial electrochemical technology

Yue Yi; Axin Liang; Lin Luo; Yuxuan Zang; Hongyu Zhao; Aiqin Luo

doi:10.1016/j.bioelechem.2021.108038

A novel real-time TMAO detection method based on microbial electrochemical technology

Yue Yi, Axin Liang, Lin Luo, Yuxuan Zang, Hongyu Zhao, Aiqin Luo^*

^*Corresponding author for this work

School of Life Science

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Trimethylamine N-oxide (TMAO) is considered to be a novel biomarker of cardiovascular diseases. However, the traditional TMAO detection method has failed to meet the requirements of real-time and point-of-care tests. Herein, a novel TMAO detection method based on microbial electrochemical technology is established, which realizes the direct conversion of TMAO concentration into electrical signals. Attached Shewanella loihica PV-4 was first proven to be capable of simultaneous inward extracellular electron transfer and TMAO reduction. The TMAO detection method showed a wide linear range of 0 to 250 μM, a high sensitivity of 23.92 μA/mM, and a low limit of detection of 5.96 μM. In addition, the TMAO detection process was accomplished within 600 s, with an acceptable accuracy of 90% in the real serum, showing high feasibility in clinical applications.

Original language	English
Article number	108038
Journal	Bioelectrochemistry
Volume	144
DOIs	https://doi.org/10.1016/j.bioelechem.2021.108038
Publication status	Published - Apr 2022

Keywords

Bioelectrochemical System
Biosensor
Electrochemically Active Bacteria
Extracellular Electron Transfer
Shewanella loihica PV-4
Trimethylamine N-oxide

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bioelechem.2021.108038

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title = "A novel real-time TMAO detection method based on microbial electrochemical technology",

abstract = "Trimethylamine N-oxide (TMAO) is considered to be a novel biomarker of cardiovascular diseases. However, the traditional TMAO detection method has failed to meet the requirements of real-time and point-of-care tests. Herein, a novel TMAO detection method based on microbial electrochemical technology is established, which realizes the direct conversion of TMAO concentration into electrical signals. Attached Shewanella loihica PV-4 was first proven to be capable of simultaneous inward extracellular electron transfer and TMAO reduction. The TMAO detection method showed a wide linear range of 0 to 250 μM, a high sensitivity of 23.92 μA/mM, and a low limit of detection of 5.96 μM. In addition, the TMAO detection process was accomplished within 600 s, with an acceptable accuracy of 90% in the real serum, showing high feasibility in clinical applications.",

keywords = "Bioelectrochemical System, Biosensor, Electrochemically Active Bacteria, Extracellular Electron Transfer, Shewanella loihica PV-4, Trimethylamine N-oxide",

author = "Yue Yi and Axin Liang and Lin Luo and Yuxuan Zang and Hongyu Zhao and Aiqin Luo",

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year = "2022",

month = apr,

doi = "10.1016/j.bioelechem.2021.108038",

language = "English",

volume = "144",

journal = "Bioelectrochemistry",

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T1 - A novel real-time TMAO detection method based on microbial electrochemical technology

AU - Yi, Yue

AU - Liang, Axin

AU - Luo, Lin

AU - Zang, Yuxuan

AU - Zhao, Hongyu

AU - Luo, Aiqin

PY - 2022/4

Y1 - 2022/4

N2 - Trimethylamine N-oxide (TMAO) is considered to be a novel biomarker of cardiovascular diseases. However, the traditional TMAO detection method has failed to meet the requirements of real-time and point-of-care tests. Herein, a novel TMAO detection method based on microbial electrochemical technology is established, which realizes the direct conversion of TMAO concentration into electrical signals. Attached Shewanella loihica PV-4 was first proven to be capable of simultaneous inward extracellular electron transfer and TMAO reduction. The TMAO detection method showed a wide linear range of 0 to 250 μM, a high sensitivity of 23.92 μA/mM, and a low limit of detection of 5.96 μM. In addition, the TMAO detection process was accomplished within 600 s, with an acceptable accuracy of 90% in the real serum, showing high feasibility in clinical applications.

AB - Trimethylamine N-oxide (TMAO) is considered to be a novel biomarker of cardiovascular diseases. However, the traditional TMAO detection method has failed to meet the requirements of real-time and point-of-care tests. Herein, a novel TMAO detection method based on microbial electrochemical technology is established, which realizes the direct conversion of TMAO concentration into electrical signals. Attached Shewanella loihica PV-4 was first proven to be capable of simultaneous inward extracellular electron transfer and TMAO reduction. The TMAO detection method showed a wide linear range of 0 to 250 μM, a high sensitivity of 23.92 μA/mM, and a low limit of detection of 5.96 μM. In addition, the TMAO detection process was accomplished within 600 s, with an acceptable accuracy of 90% in the real serum, showing high feasibility in clinical applications.

KW - Bioelectrochemical System

KW - Biosensor

KW - Electrochemically Active Bacteria

KW - Extracellular Electron Transfer

KW - Shewanella loihica PV-4

KW - Trimethylamine N-oxide

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DO - 10.1016/j.bioelechem.2021.108038

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SN - 1567-5394

VL - 144

JO - Bioelectrochemistry

JF - Bioelectrochemistry

M1 - 108038

ER -

A novel real-time TMAO detection method based on microbial electrochemical technology

Abstract

Keywords

UN SDGs

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