TY - JOUR
T1 - A novel real-time TMAO detection method based on microbial electrochemical technology
AU - Yi, Yue
AU - Liang, Axin
AU - Luo, Lin
AU - Zang, Yuxuan
AU - Zhao, Hongyu
AU - Luo, Aiqin
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/4
Y1 - 2022/4
N2 - Trimethylamine N-oxide (TMAO) is considered to be a novel biomarker of cardiovascular diseases. However, the traditional TMAO detection method has failed to meet the requirements of real-time and point-of-care tests. Herein, a novel TMAO detection method based on microbial electrochemical technology is established, which realizes the direct conversion of TMAO concentration into electrical signals. Attached Shewanella loihica PV-4 was first proven to be capable of simultaneous inward extracellular electron transfer and TMAO reduction. The TMAO detection method showed a wide linear range of 0 to 250 μM, a high sensitivity of 23.92 μA/mM, and a low limit of detection of 5.96 μM. In addition, the TMAO detection process was accomplished within 600 s, with an acceptable accuracy of 90% in the real serum, showing high feasibility in clinical applications.
AB - Trimethylamine N-oxide (TMAO) is considered to be a novel biomarker of cardiovascular diseases. However, the traditional TMAO detection method has failed to meet the requirements of real-time and point-of-care tests. Herein, a novel TMAO detection method based on microbial electrochemical technology is established, which realizes the direct conversion of TMAO concentration into electrical signals. Attached Shewanella loihica PV-4 was first proven to be capable of simultaneous inward extracellular electron transfer and TMAO reduction. The TMAO detection method showed a wide linear range of 0 to 250 μM, a high sensitivity of 23.92 μA/mM, and a low limit of detection of 5.96 μM. In addition, the TMAO detection process was accomplished within 600 s, with an acceptable accuracy of 90% in the real serum, showing high feasibility in clinical applications.
KW - Bioelectrochemical System
KW - Biosensor
KW - Electrochemically Active Bacteria
KW - Extracellular Electron Transfer
KW - Shewanella loihica PV-4
KW - Trimethylamine N-oxide
UR - http://www.scopus.com/inward/record.url?scp=85120993207&partnerID=8YFLogxK
U2 - 10.1016/j.bioelechem.2021.108038
DO - 10.1016/j.bioelechem.2021.108038
M3 - Article
C2 - 34906816
AN - SCOPUS:85120993207
SN - 1567-5394
VL - 144
JO - Bioelectrochemistry
JF - Bioelectrochemistry
M1 - 108038
ER -
