摘要
This paper developed a microfluidic chip for whole genome sequencing (WGS) of circulating tumor cells (CTC) at single-cell level. For the first time, all processing steps between collecting blood sample and feeding prepared sample to sequencer are finished in a single microfluidic chips. The microfluidic chip comprehensively performs blood filtering, CTC enrichment, CTC identification/isolation, CTC lysis and whole genome amplification (WGA) at the single cell level. The microfluidic chip was validated by clinical samples. Our results demonstrate that for the first time, the aim of 'whole blood in, WGA product out' has been realized at single CTC level with clinical samples, in a microfluidic chip.
| 源语言 | 英语 |
|---|---|
| 主期刊名 | 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019 |
| 出版商 | Institute of Electrical and Electronics Engineers Inc. |
| 页 | 412-415 |
| 页数 | 4 |
| ISBN（电子版） | 9781728116105 |
| DOI | |
| 出版状态 | 已出版 - 1月 2019 |
| 已对外发布 | 是 |
| 活动 | 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 - Seoul, 韩国 期限: 27 1月 2019 → 31 1月 2019 |
出版系列
| 姓名 | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
|---|---|
| 卷 | 2019-January |
| ISSN（印刷版） | 1084-6999 |
会议
| 会议 | 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 |
|---|---|
| 国家/地区 | 韩国 |
| 市 | Seoul |
| 时期 | 27/01/19 → 31/01/19 |
指纹
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Ren, L., Fei, J., Zhang, W. K., Fang, Z. G., Hu, Z. Y., & Wei, Z. W. (2019). A Microfluidic Chip for CTC Whole Genome Sequencing. 在 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019 (页码 412-415). 文章 8870769 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); 卷 2019-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2019.8870769