A Microfluidic Chip for CTC Whole Genome Sequencing

Li Ren; Jia Fei; W. K. Zhang; Z. G. Fang; Z. Y. Hu; Z. W. Wei

doi:10.1109/MEMSYS.2019.8870769

A Microfluidic Chip for CTC Whole Genome Sequencing

Li Ren, Jia Fei, W. K. Zhang, Z. G. Fang, Z. Y. Hu, Z. W. Wei

National Center for Nanoscience and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

This paper developed a microfluidic chip for whole genome sequencing (WGS) of circulating tumor cells (CTC) at single-cell level. For the first time, all processing steps between collecting blood sample and feeding prepared sample to sequencer are finished in a single microfluidic chips. The microfluidic chip comprehensively performs blood filtering, CTC enrichment, CTC identification/isolation, CTC lysis and whole genome amplification (WGA) at the single cell level. The microfluidic chip was validated by clinical samples. Our results demonstrate that for the first time, the aim of 'whole blood in, WGA product out' has been realized at single CTC level with clinical samples, in a microfluidic chip.

Original language	English
Title of host publication	2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	412-415
Number of pages	4
ISBN (Electronic)	9781728116105
DOIs	https://doi.org/10.1109/MEMSYS.2019.8870769
Publication status	Published - Jan 2019
Externally published	Yes
Event	32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 - Seoul, Korea, Republic of Duration: 27 Jan 2019 → 31 Jan 2019

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2019-January
ISSN (Print)	1084-6999

Conference

Conference	32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019
Country/Territory	Korea, Republic of
City	Seoul
Period	27/01/19 → 31/01/19

Access to Document

10.1109/MEMSYS.2019.8870769

Cite this

Ren, L., Fei, J., Zhang, W. K., Fang, Z. G., Hu, Z. Y., & Wei, Z. W. (2019). A Microfluidic Chip for CTC Whole Genome Sequencing. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019 (pp. 412-415). Article 8870769 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2019.8870769

@inproceedings{0f7ab2ab65e34c1991f5e9f092cce061,

title = "A Microfluidic Chip for CTC Whole Genome Sequencing",

abstract = "This paper developed a microfluidic chip for whole genome sequencing (WGS) of circulating tumor cells (CTC) at single-cell level. For the first time, all processing steps between collecting blood sample and feeding prepared sample to sequencer are finished in a single microfluidic chips. The microfluidic chip comprehensively performs blood filtering, CTC enrichment, CTC identification/isolation, CTC lysis and whole genome amplification (WGA) at the single cell level. The microfluidic chip was validated by clinical samples. Our results demonstrate that for the first time, the aim of 'whole blood in, WGA product out' has been realized at single CTC level with clinical samples, in a microfluidic chip.",

author = "Li Ren and Jia Fei and Zhang, {W. K.} and Fang, {Z. G.} and Hu, {Z. Y.} and Wei, {Z. W.}",

note = "Publisher Copyright: {\textcopyright} 2019 IEEE.; 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 ; Conference date: 27-01-2019 Through 31-01-2019",

year = "2019",

month = jan,

doi = "10.1109/MEMSYS.2019.8870769",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "412--415",

booktitle = "2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019",

address = "United States",

}

Ren, L, Fei, J, Zhang, WK, Fang, ZG, Hu, ZY & Wei, ZW 2019, A Microfluidic Chip for CTC Whole Genome Sequencing. in 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019., 8870769, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2019-January, Institute of Electrical and Electronics Engineers Inc., pp. 412-415, 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019, Seoul, Korea, Republic of, 27/01/19. https://doi.org/10.1109/MEMSYS.2019.8870769

A Microfluidic Chip for CTC Whole Genome Sequencing. / Ren, Li; Fei, Jia; Zhang, W. K. et al.
2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc., 2019. p. 412-415 8870769 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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N2 - This paper developed a microfluidic chip for whole genome sequencing (WGS) of circulating tumor cells (CTC) at single-cell level. For the first time, all processing steps between collecting blood sample and feeding prepared sample to sequencer are finished in a single microfluidic chips. The microfluidic chip comprehensively performs blood filtering, CTC enrichment, CTC identification/isolation, CTC lysis and whole genome amplification (WGA) at the single cell level. The microfluidic chip was validated by clinical samples. Our results demonstrate that for the first time, the aim of 'whole blood in, WGA product out' has been realized at single CTC level with clinical samples, in a microfluidic chip.

AB - This paper developed a microfluidic chip for whole genome sequencing (WGS) of circulating tumor cells (CTC) at single-cell level. For the first time, all processing steps between collecting blood sample and feeding prepared sample to sequencer are finished in a single microfluidic chips. The microfluidic chip comprehensively performs blood filtering, CTC enrichment, CTC identification/isolation, CTC lysis and whole genome amplification (WGA) at the single cell level. The microfluidic chip was validated by clinical samples. Our results demonstrate that for the first time, the aim of 'whole blood in, WGA product out' has been realized at single CTC level with clinical samples, in a microfluidic chip.

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Ren L, Fei J, Zhang WK, Fang ZG, Hu ZY, Wei ZW. A Microfluidic Chip for CTC Whole Genome Sequencing. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc. 2019. p. 412-415. 8870769. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2019.8870769

A Microfluidic Chip for CTC Whole Genome Sequencing

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