TY - JOUR
T1 - Two-step separation-free quantitative detection of HSA and FIB in human blood plasma by a pentaphenylpyyrrole derivative with aggregation-enhanced emission properties
AU - Chen, Didi
AU - Dong, Lichao
AU - Jiang, Shuai
AU - Li, Wangyang
AU - Shi, Jianbing
AU - Feng, Xiao
AU - Zhi, Junge
AU - Tong, Bin
AU - Li, Min
AU - Zheng, Qichang
AU - Dong, Yuping
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/2
Y1 - 2018/2
N2 - A novel fluorescence based strategy, employing the aggregation-enhanced emission (AEE) property of sodium 4,4′,4”-(3,4-diphenyl-1H-pyrrole-1,2,5-triyl)tribenzoate (DP-TPPNa), was proposed to determine the concentrations of human serum albumin (HSA) and fibrinogen (FIB) in human plasma without separation required. HSA level was determined at concentrations lower than 110 μg/mL by the selective PL response of DP-TPPNa to HSA and FIB was quantified at high HSA concentrations in the range of 140–200 μg/mL by the synergistic effects of FIB and HSA on the PL intensity of DP-TPPNa. Compared with authoritative instruments, the method developed in the present work is rapid, simple, extremely sensitive, highly selective and precise for the quantitative detection of proteins with one single system, which is an ideal detection system candidate for the early diagnosis of diseases. The results also indicate that the interactions between proteins can affect their configuration and conformation blood plasma. This can be used to study the correlations among the serum components.
AB - A novel fluorescence based strategy, employing the aggregation-enhanced emission (AEE) property of sodium 4,4′,4”-(3,4-diphenyl-1H-pyrrole-1,2,5-triyl)tribenzoate (DP-TPPNa), was proposed to determine the concentrations of human serum albumin (HSA) and fibrinogen (FIB) in human plasma without separation required. HSA level was determined at concentrations lower than 110 μg/mL by the selective PL response of DP-TPPNa to HSA and FIB was quantified at high HSA concentrations in the range of 140–200 μg/mL by the synergistic effects of FIB and HSA on the PL intensity of DP-TPPNa. Compared with authoritative instruments, the method developed in the present work is rapid, simple, extremely sensitive, highly selective and precise for the quantitative detection of proteins with one single system, which is an ideal detection system candidate for the early diagnosis of diseases. The results also indicate that the interactions between proteins can affect their configuration and conformation blood plasma. This can be used to study the correlations among the serum components.
KW - Aggregation-enhanced emission
KW - Fibrinogen
KW - Pentaphenylpyyrrole
KW - Serum albumin
KW - Synergistic effect
UR - http://www.scopus.com/inward/record.url?scp=85028429238&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2017.08.142
DO - 10.1016/j.snb.2017.08.142
M3 - Article
AN - SCOPUS:85028429238
SN - 0925-4005
VL - 255
SP - 854
EP - 861
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -
