TY - JOUR
T1 - Three-dimensional resolution-enhancement divided aperture correlation-differential confocal microscopy with nanometer axial focusing capability
AU - Qiu, Lirong
AU - Wang, Yun
AU - Wu, Hanxu
AU - Sun, Yingbin
AU - Cui, Han
AU - Zhao, Weiqian
AU - Yuan, Liang
AU - Zhan, Chunlian
N1 - Publisher Copyright:
© 2018 Optical Society of America.
PY - 2018/2/5
Y1 - 2018/2/5
N2 - Divided aperture confocal microscopy (DACM) provides an improved imaging depth, imaging contrast, and working distance at the expense of spatial resolution. Here, we present a new method-divided aperture correlation-differential confocal microscopy (DACDCM) to improve the DACM resolution and the focusing capability, without changing the DACM configuration. DACDCM divides the DACM image spot into two round regions symmetrical about the optical axis. Then the light intensity signals received simultaneously from two round regions by a charge-coupled device (CCD) are processed by correlation manipulation and differential subtraction to improve the DACM spatial resolution and axial focusing capability, respectively. Theoretical analysis and preliminary experiments indicate that, for the excitation wavelength of λ=632.8 nm, numerical aperture NA=0.8, and normalized offset vM=3.2 of the two regions, the DACDCM resolution is improved by 32.5% and 43.1% in the x and z directions, simultaneously, compared with that of the DACM. The axial focusing resolution used for the sample surface profile imaging was also significantly improved to 2 nm.
AB - Divided aperture confocal microscopy (DACM) provides an improved imaging depth, imaging contrast, and working distance at the expense of spatial resolution. Here, we present a new method-divided aperture correlation-differential confocal microscopy (DACDCM) to improve the DACM resolution and the focusing capability, without changing the DACM configuration. DACDCM divides the DACM image spot into two round regions symmetrical about the optical axis. Then the light intensity signals received simultaneously from two round regions by a charge-coupled device (CCD) are processed by correlation manipulation and differential subtraction to improve the DACM spatial resolution and axial focusing capability, respectively. Theoretical analysis and preliminary experiments indicate that, for the excitation wavelength of λ=632.8 nm, numerical aperture NA=0.8, and normalized offset vM=3.2 of the two regions, the DACDCM resolution is improved by 32.5% and 43.1% in the x and z directions, simultaneously, compared with that of the DACM. The axial focusing resolution used for the sample surface profile imaging was also significantly improved to 2 nm.
UR - http://www.scopus.com/inward/record.url?scp=85041473814&partnerID=8YFLogxK
U2 - 10.1364/OE.26.002314
DO - 10.1364/OE.26.002314
M3 - Article
C2 - 29401771
AN - SCOPUS:85041473814
SN - 1094-4087
VL - 26
SP - 2314
EP - 2324
JO - Optics Express
JF - Optics Express
IS - 3
ER -