Specific Two-Photon Imaging of Live Cellular and Deep-Tissue Lipid Droplets by Lipophilic AIEgens at Ultralow Concentration

Guangle Niu; Ruoyao Zhang; John P.C. Kwong; Jacky W.Y. Lam; Congping Chen; Jianguo Wang; Yuncong Chen; Xing Feng; Ryan T.K. Kwok; Herman H.Y. Sung; Ian D. Williams; Mark R.J. Elsegood; Jianan Qu; Chao Ma; Kam Sing Wong; Xiaoqiang Yu; Ben Zhong Tang

doi:10.1021/acs.chemmater.8b01943

Specific Two-Photon Imaging of Live Cellular and Deep-Tissue Lipid Droplets by Lipophilic AIEgens at Ultralow Concentration

Guangle Niu, Ruoyao Zhang, John P.C. Kwong, Jacky W.Y. Lam, Congping Chen, Jianguo Wang, Yuncong Chen, Xing Feng, Ryan T.K. Kwok, Herman H.Y. Sung, Ian D. Williams, Mark R.J. Elsegood, Jianan Qu, Chao Ma, Kam Sing Wong, Xiaoqiang Yu^*, Ben Zhong Tang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

161 Citations (Scopus)

Abstract

Lipid droplets are highly associated with obesity, diabetes, inflammatory disorders, and cancer. A reliable two-photon dye for specific lipid droplets imaging in live cells and live tissues at ultralow concentration has rarely been reported. In this work, four new aggregation-induced emission luminogens (AIEgens) based on the naphthalene core were designed and synthesized for specific two-photon lipid droplet staining. The new molecules, namely, NAP AIEgens, exhibit large Stokes shift (>110 nm), high solid-state fluorescence quantum yield (up to 30%), good two-photon absorption cross section (45-100 GM at 860 nm), high biocompatibility, and good photostability. They could specifically stain lipid droplets at ultralow concentration (50 nM) in a short time of 15 min. Such ultralow concentration is the lowest value for lipid droplets staining in live cells reported so far. In vitro and ex vivo two-photon imaging of lipid droplets in live cells and live mice liver tissues were successfully demonstrated. In addition, selective visualization of lipid droplets in live mice liver tissues could be achieved at a depth of about 70 μm. These excellent properties render them as promising candidates for investigating lipid droplet-associated physiological and pathological processes in live biological samples.

Original language	English
Pages (from-to)	4778-4787
Number of pages	10
Journal	Chemistry of Materials
Volume	30
Issue number	14
DOIs	https://doi.org/10.1021/acs.chemmater.8b01943
Publication status	Published - 24 Jul 2018
Externally published	Yes

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Niu, G., Zhang, R., Kwong, J. P. C., Lam, J. W. Y., Chen, C., Wang, J., Chen, Y., Feng, X., Kwok, R. T. K., Sung, H. H. Y., Williams, I. D., Elsegood, M. R. J., Qu, J., Ma, C., Wong, K. S., Yu, X., & Tang, B. Z. (2018). Specific Two-Photon Imaging of Live Cellular and Deep-Tissue Lipid Droplets by Lipophilic AIEgens at Ultralow Concentration. Chemistry of Materials, 30(14), 4778-4787. https://doi.org/10.1021/acs.chemmater.8b01943

@article{37783eebdc9749e99eaaef62b998ced5,

title = "Specific Two-Photon Imaging of Live Cellular and Deep-Tissue Lipid Droplets by Lipophilic AIEgens at Ultralow Concentration",

abstract = "Lipid droplets are highly associated with obesity, diabetes, inflammatory disorders, and cancer. A reliable two-photon dye for specific lipid droplets imaging in live cells and live tissues at ultralow concentration has rarely been reported. In this work, four new aggregation-induced emission luminogens (AIEgens) based on the naphthalene core were designed and synthesized for specific two-photon lipid droplet staining. The new molecules, namely, NAP AIEgens, exhibit large Stokes shift (>110 nm), high solid-state fluorescence quantum yield (up to 30%), good two-photon absorption cross section (45-100 GM at 860 nm), high biocompatibility, and good photostability. They could specifically stain lipid droplets at ultralow concentration (50 nM) in a short time of 15 min. Such ultralow concentration is the lowest value for lipid droplets staining in live cells reported so far. In vitro and ex vivo two-photon imaging of lipid droplets in live cells and live mice liver tissues were successfully demonstrated. In addition, selective visualization of lipid droplets in live mice liver tissues could be achieved at a depth of about 70 μm. These excellent properties render them as promising candidates for investigating lipid droplet-associated physiological and pathological processes in live biological samples.",

author = "Guangle Niu and Ruoyao Zhang and Kwong, {John P.C.} and Lam, {Jacky W.Y.} and Congping Chen and Jianguo Wang and Yuncong Chen and Xing Feng and Kwok, {Ryan T.K.} and Sung, {Herman H.Y.} and Williams, {Ian D.} and Elsegood, {Mark R.J.} and Jianan Qu and Chao Ma and Wong, {Kam Sing} and Xiaoqiang Yu and Tang, {Ben Zhong}",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = jul,

day = "24",

doi = "10.1021/acs.chemmater.8b01943",

language = "English",

volume = "30",

pages = "4778--4787",

journal = "Chemistry of Materials",

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Niu, G , Zhang, R, Kwong, JPC, Lam, JWY, Chen, C, Wang, J, Chen, Y, Feng, X, Kwok, RTK, Sung, HHY, Williams, ID, Elsegood, MRJ, Qu, J, Ma, C, Wong, KS, Yu, X & Tang, BZ 2018, 'Specific Two-Photon Imaging of Live Cellular and Deep-Tissue Lipid Droplets by Lipophilic AIEgens at Ultralow Concentration', Chemistry of Materials, vol. 30, no. 14, pp. 4778-4787. https://doi.org/10.1021/acs.chemmater.8b01943

TY - JOUR

T1 - Specific Two-Photon Imaging of Live Cellular and Deep-Tissue Lipid Droplets by Lipophilic AIEgens at Ultralow Concentration

AU - Niu, Guangle

AU - Zhang, Ruoyao

AU - Kwong, John P.C.

AU - Lam, Jacky W.Y.

AU - Chen, Congping

AU - Wang, Jianguo

AU - Chen, Yuncong

AU - Feng, Xing

AU - Kwok, Ryan T.K.

AU - Sung, Herman H.Y.

AU - Williams, Ian D.

AU - Elsegood, Mark R.J.

AU - Qu, Jianan

AU - Ma, Chao

AU - Wong, Kam Sing

AU - Yu, Xiaoqiang

AU - Tang, Ben Zhong

PY - 2018/7/24

Y1 - 2018/7/24

N2 - Lipid droplets are highly associated with obesity, diabetes, inflammatory disorders, and cancer. A reliable two-photon dye for specific lipid droplets imaging in live cells and live tissues at ultralow concentration has rarely been reported. In this work, four new aggregation-induced emission luminogens (AIEgens) based on the naphthalene core were designed and synthesized for specific two-photon lipid droplet staining. The new molecules, namely, NAP AIEgens, exhibit large Stokes shift (>110 nm), high solid-state fluorescence quantum yield (up to 30%), good two-photon absorption cross section (45-100 GM at 860 nm), high biocompatibility, and good photostability. They could specifically stain lipid droplets at ultralow concentration (50 nM) in a short time of 15 min. Such ultralow concentration is the lowest value for lipid droplets staining in live cells reported so far. In vitro and ex vivo two-photon imaging of lipid droplets in live cells and live mice liver tissues were successfully demonstrated. In addition, selective visualization of lipid droplets in live mice liver tissues could be achieved at a depth of about 70 μm. These excellent properties render them as promising candidates for investigating lipid droplet-associated physiological and pathological processes in live biological samples.

AB - Lipid droplets are highly associated with obesity, diabetes, inflammatory disorders, and cancer. A reliable two-photon dye for specific lipid droplets imaging in live cells and live tissues at ultralow concentration has rarely been reported. In this work, four new aggregation-induced emission luminogens (AIEgens) based on the naphthalene core were designed and synthesized for specific two-photon lipid droplet staining. The new molecules, namely, NAP AIEgens, exhibit large Stokes shift (>110 nm), high solid-state fluorescence quantum yield (up to 30%), good two-photon absorption cross section (45-100 GM at 860 nm), high biocompatibility, and good photostability. They could specifically stain lipid droplets at ultralow concentration (50 nM) in a short time of 15 min. Such ultralow concentration is the lowest value for lipid droplets staining in live cells reported so far. In vitro and ex vivo two-photon imaging of lipid droplets in live cells and live mice liver tissues were successfully demonstrated. In addition, selective visualization of lipid droplets in live mice liver tissues could be achieved at a depth of about 70 μm. These excellent properties render them as promising candidates for investigating lipid droplet-associated physiological and pathological processes in live biological samples.

UR - http://www.scopus.com/inward/record.url?scp=85049633728&partnerID=8YFLogxK

U2 - 10.1021/acs.chemmater.8b01943

DO - 10.1021/acs.chemmater.8b01943

M3 - Article

AN - SCOPUS:85049633728

SN - 0897-4756

VL - 30

SP - 4778

EP - 4787

JO - Chemistry of Materials

JF - Chemistry of Materials

IS - 14

ER -

Specific Two-Photon Imaging of Live Cellular and Deep-Tissue Lipid Droplets by Lipophilic AIEgens at Ultralow Concentration

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