Cui, L., Lin, S., Yang, X., Xie, X., Wang, X., He, N., Yang, J., Zhang, X., Lu, X., Yan, X., Guo, Y., Zhang, B., Li, R., Miao, H., Ji, M., Zhang, R., Yu, L., Xiao, Z., Wei, Y., & Guo, J. (Accepted/In press). Spatial transcriptomic characterization of a Carnegie stage 7 human embryo. Nature Cell Biology, Article e13298. https://doi.org/10.1038/s41556-024-01597-3
Cui, Lina ; Lin, Sirui ; Yang, Xiaolong et al. / Spatial transcriptomic characterization of a Carnegie stage 7 human embryo. In: Nature Cell Biology. 2025.
@article{07f8841b8363424ca2ac07a79b1ec390,
title = "Spatial transcriptomic characterization of a Carnegie stage 7 human embryo",
abstract = "Gastrulation marks a pivotal stage in mammalian embryonic development, establishing the three germ layers and body axis through lineage diversification and morphogenetic movements. However, studying human gastrulating embryos is challenging due to limited access to early tissues. Here we show the use of spatial transcriptomics to analyse a fully intact Carnegie stage 7 human embryo at single-cell resolution, along with immunofluorescence validations in a second embryo. Employing 82 serial cryosections and Stereo-seq technology, we reconstructed a three-dimensional model of the embryo. Our findings reveal early specification of distinct mesoderm subtypes and the presence of the anterior visceral endoderm. Notably, primordial germ cells were located in the connecting stalk, and haematopoietic stem cell-independent haematopoiesis was observed in the yolk sac. This study advances our understanding of human gastrulation and provides a valuable dataset for future research in early human development.",
author = "Lina Cui and Sirui Lin and Xiaolong Yang and Xinwei Xie and Xiaoyan Wang and Nannan He and Jingyu Yang and Xin Zhang and Xiaojian Lu and Xiaodi Yan and Yifei Guo and Bailing Zhang and Ran Li and Hefan Miao and Mei Ji and Runzhao Zhang and Leqian Yu and Zhenyu Xiao and Yulei Wei and Jingtao Guo",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive licence to Springer Nature Limited 2025.",
year = "2025",
doi = "10.1038/s41556-024-01597-3",
language = "English",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Research",
}
Cui, L, Lin, S, Yang, X, Xie, X, Wang, X, He, N, Yang, J, Zhang, X, Lu, X, Yan, X, Guo, Y, Zhang, B, Li, R, Miao, H, Ji, M, Zhang, R, Yu, L, Xiao, Z, Wei, Y & Guo, J 2025, 'Spatial transcriptomic characterization of a Carnegie stage 7 human embryo', Nature Cell Biology. https://doi.org/10.1038/s41556-024-01597-3
Spatial transcriptomic characterization of a Carnegie stage 7 human embryo. / Cui, Lina; Lin, Sirui; Yang, Xiaolong et al.
In:
Nature Cell Biology, 2025.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Spatial transcriptomic characterization of a Carnegie stage 7 human embryo
AU - Cui, Lina
AU - Lin, Sirui
AU - Yang, Xiaolong
AU - Xie, Xinwei
AU - Wang, Xiaoyan
AU - He, Nannan
AU - Yang, Jingyu
AU - Zhang, Xin
AU - Lu, Xiaojian
AU - Yan, Xiaodi
AU - Guo, Yifei
AU - Zhang, Bailing
AU - Li, Ran
AU - Miao, Hefan
AU - Ji, Mei
AU - Zhang, Runzhao
AU - Yu, Leqian
AU - Xiao, Zhenyu
AU - Wei, Yulei
AU - Guo, Jingtao
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Nature Limited 2025.
PY - 2025
Y1 - 2025
N2 - Gastrulation marks a pivotal stage in mammalian embryonic development, establishing the three germ layers and body axis through lineage diversification and morphogenetic movements. However, studying human gastrulating embryos is challenging due to limited access to early tissues. Here we show the use of spatial transcriptomics to analyse a fully intact Carnegie stage 7 human embryo at single-cell resolution, along with immunofluorescence validations in a second embryo. Employing 82 serial cryosections and Stereo-seq technology, we reconstructed a three-dimensional model of the embryo. Our findings reveal early specification of distinct mesoderm subtypes and the presence of the anterior visceral endoderm. Notably, primordial germ cells were located in the connecting stalk, and haematopoietic stem cell-independent haematopoiesis was observed in the yolk sac. This study advances our understanding of human gastrulation and provides a valuable dataset for future research in early human development.
AB - Gastrulation marks a pivotal stage in mammalian embryonic development, establishing the three germ layers and body axis through lineage diversification and morphogenetic movements. However, studying human gastrulating embryos is challenging due to limited access to early tissues. Here we show the use of spatial transcriptomics to analyse a fully intact Carnegie stage 7 human embryo at single-cell resolution, along with immunofluorescence validations in a second embryo. Employing 82 serial cryosections and Stereo-seq technology, we reconstructed a three-dimensional model of the embryo. Our findings reveal early specification of distinct mesoderm subtypes and the presence of the anterior visceral endoderm. Notably, primordial germ cells were located in the connecting stalk, and haematopoietic stem cell-independent haematopoiesis was observed in the yolk sac. This study advances our understanding of human gastrulation and provides a valuable dataset for future research in early human development.
UR - http://www.scopus.com/inward/record.url?scp=85217167386&partnerID=8YFLogxK
U2 - 10.1038/s41556-024-01597-3
DO - 10.1038/s41556-024-01597-3
M3 - Article
C2 - 39794460
AN - SCOPUS:85217167386
SN - 1465-7392
JO - Nature Cell Biology
JF - Nature Cell Biology
M1 - e13298
ER -
Cui L, Lin S, Yang X, Xie X, Wang X, He N et al. Spatial transcriptomic characterization of a Carnegie stage 7 human embryo. Nature Cell Biology. 2025;e13298. doi: 10.1038/s41556-024-01597-3
