Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH

Rongji Dai; Jie Chen; Jie Lin; Shenyuan Xiao; Shi Chen; Yulin Deng

doi:10.1016/j.jhazmat.2009.04.122

Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH

Rongji Dai^*, Jie Chen, Jie Lin, Shenyuan Xiao, Shi Chen, Yulin Deng

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49% when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28% when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08% when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme.

Original language	English
Pages (from-to)	141-143
Number of pages	3
Journal	Journal of Hazardous Materials
Volume	170
Issue number	1
DOIs	https://doi.org/10.1016/j.jhazmat.2009.04.122
Publication status	Published - 15 Oct 2009

Keywords

Nitrophenols
Nitroreductase
Reduction

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10.1016/j.jhazmat.2009.04.122

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Dai, R., Chen, J., Lin, J., Xiao, S., Chen, S., & Deng, Y. (2009). Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH. Journal of Hazardous Materials, 170(1), 141-143. https://doi.org/10.1016/j.jhazmat.2009.04.122

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title = "Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH",

abstract = "The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49% when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28% when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08% when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme.",

keywords = "Nitrophenols, Nitroreductase, Reduction",

author = "Rongji Dai and Jie Chen and Jie Lin and Shenyuan Xiao and Shi Chen and Yulin Deng",

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T1 - Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH

AU - Dai, Rongji

AU - Chen, Jie

AU - Lin, Jie

AU - Xiao, Shenyuan

AU - Chen, Shi

AU - Deng, Yulin

PY - 2009/10/15

Y1 - 2009/10/15

N2 - The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49% when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28% when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08% when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme.

AB - The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49% when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28% when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08% when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme.

KW - Nitrophenols

KW - Nitroreductase

KW - Reduction

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AN - SCOPUS:67651111846

SN - 0304-3894

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SP - 141

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JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

IS - 1

ER -

Reduction of nitro phenols using nitroreductase from E. coli in the presence of NADH

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Keywords

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