Abstract
The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49% when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28% when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08% when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme.
| Original language | English |
|---|---|
| Pages (from-to) | 141-143 |
| Number of pages | 3 |
| Journal | Journal of Hazardous Materials |
| Volume | 170 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 15 Oct 2009 |
Keywords
- Nitrophenols
- Nitroreductase
- Reduction