Radiation enhance biological activity of recombinant adeno-associated virus against human glioma through down-regulating ATM

Zuye Yang, Yunjia Zhang, Zhaoying Fu, Hailong Wang, Hong Ma*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Object Whether radiation can improve the biological activity of recombinant adeno-associated virus (rAAV) in U251 cells and the molecule mechanism. Methods 60Co as the radiation resource to irradiate tumor cells; Inverted fluorescence microscope was performed to observe expression of EGFP; flow cytometry (FACS) analysis for transduction efficiency and mean fluorescent intensity; RT-PCR analysis for mRNA expression of rAAV binding receptors genes-HSPG, FGFR1 and Integrin, and DNA circularization related gene-ATM. Results Transduction efficiency don't have a notable change after radiation. But the mean fluorescent intensity was improved with the rise of radiation dose. In mRNA expression level, the binding receptors for rAAV targeting cells, as well as ATM, have down-regulated. Conclusion Radiation can enhance biological activity of rAAV, and the enhance effect is not related to the 'rAAV binding receptors' procedure. But down-regulation of ATM might affect this process.

Original languageEnglish
Title of host publication2013 ICME International Conference on Complex Medical Engineering, CME 2013
Pages165-168
Number of pages4
DOIs
Publication statusPublished - 2013
Event2013 7th ICME International Conference on Complex Medical Engineering, CME 2013 - Beijing, China
Duration: 25 May 201328 May 2013

Publication series

Name2013 ICME International Conference on Complex Medical Engineering, CME 2013

Conference

Conference2013 7th ICME International Conference on Complex Medical Engineering, CME 2013
Country/TerritoryChina
CityBeijing
Period25/05/1328/05/13

Keywords

  • ATM
  • biological activity
  • radiation
  • recombinant adeno-associated virus

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Yang, Z., Zhang, Y., Fu, Z., Wang, H., & Ma, H. (2013). Radiation enhance biological activity of recombinant adeno-associated virus against human glioma through down-regulating ATM. In 2013 ICME International Conference on Complex Medical Engineering, CME 2013 (pp. 165-168). Article 6548232 (2013 ICME International Conference on Complex Medical Engineering, CME 2013). https://doi.org/10.1109/ICCME.2013.6548232