TY - GEN
T1 - Radiation enhance biological activity of recombinant adeno-associated virus against human glioma through down-regulating ATM
AU - Yang, Zuye
AU - Zhang, Yunjia
AU - Fu, Zhaoying
AU - Wang, Hailong
AU - Ma, Hong
PY - 2013
Y1 - 2013
N2 - Object Whether radiation can improve the biological activity of recombinant adeno-associated virus (rAAV) in U251 cells and the molecule mechanism. Methods 60Co as the radiation resource to irradiate tumor cells; Inverted fluorescence microscope was performed to observe expression of EGFP; flow cytometry (FACS) analysis for transduction efficiency and mean fluorescent intensity; RT-PCR analysis for mRNA expression of rAAV binding receptors genes-HSPG, FGFR1 and Integrin, and DNA circularization related gene-ATM. Results Transduction efficiency don't have a notable change after radiation. But the mean fluorescent intensity was improved with the rise of radiation dose. In mRNA expression level, the binding receptors for rAAV targeting cells, as well as ATM, have down-regulated. Conclusion Radiation can enhance biological activity of rAAV, and the enhance effect is not related to the 'rAAV binding receptors' procedure. But down-regulation of ATM might affect this process.
AB - Object Whether radiation can improve the biological activity of recombinant adeno-associated virus (rAAV) in U251 cells and the molecule mechanism. Methods 60Co as the radiation resource to irradiate tumor cells; Inverted fluorescence microscope was performed to observe expression of EGFP; flow cytometry (FACS) analysis for transduction efficiency and mean fluorescent intensity; RT-PCR analysis for mRNA expression of rAAV binding receptors genes-HSPG, FGFR1 and Integrin, and DNA circularization related gene-ATM. Results Transduction efficiency don't have a notable change after radiation. But the mean fluorescent intensity was improved with the rise of radiation dose. In mRNA expression level, the binding receptors for rAAV targeting cells, as well as ATM, have down-regulated. Conclusion Radiation can enhance biological activity of rAAV, and the enhance effect is not related to the 'rAAV binding receptors' procedure. But down-regulation of ATM might affect this process.
KW - ATM
KW - biological activity
KW - radiation
KW - recombinant adeno-associated virus
UR - http://www.scopus.com/inward/record.url?scp=84881520370&partnerID=8YFLogxK
U2 - 10.1109/ICCME.2013.6548232
DO - 10.1109/ICCME.2013.6548232
M3 - Conference contribution
AN - SCOPUS:84881520370
SN - 9781467329699
T3 - 2013 ICME International Conference on Complex Medical Engineering, CME 2013
SP - 165
EP - 168
BT - 2013 ICME International Conference on Complex Medical Engineering, CME 2013
T2 - 2013 7th ICME International Conference on Complex Medical Engineering, CME 2013
Y2 - 25 May 2013 through 28 May 2013
ER -