Production of the lipase from penicillium expansum PED-03 and its application in the resolution of racemic allethrolone

Da Zhang Dai*, Li Ming Xia

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The resolution of racemic allethrolone (4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-one) catalyzed by lipase instead of chemical methods was investigated. A high-yield lipase producing strain Penicillium expansum PED-03 was obtained by Rhodamine B plate screen. It is suitable for the lipase production with 0.5% starch as carbon source and 4.0% soybean meal as nitrogen source in submerged fermentation. Tween-80 is an effective enhancer for lipase production, and the lipase activity can be increased 55% when 0.05% Tween-80 was added to the medium. Agitation speed plays an important role in the lipase production, and the suitable agitation speed is 500 r·min-1. The resolution of racemic allethrolone catalyzed by the lipase from P. expansum PED-03 was carried out in a nonaqueous medium, and after reacting for 36 hours, the conversion reaches 96% of its theoretical value and the enantiomeric excess of product (eep) reaches 99%. It shows that the enzymatic resolution method proposed presents a good promise of using it for the reslution of racemic allethrolone.

Original languageEnglish
Pages (from-to)166-171
Number of pages6
JournalGao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities
Volume22
Issue number1
Publication statusPublished - Feb 2008
Externally publishedYes

Keywords

  • Allethrolone
  • Enzymatic resolution
  • Lipase
  • Submerged fermentation

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Dai, D. Z., & Xia, L. M. (2008). Production of the lipase from penicillium expansum PED-03 and its application in the resolution of racemic allethrolone. Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities, 22(1), 166-171.