Optimization of biodegradation of dibenzothiophene using response surface methodology

Zhi Guo Guo, Jian Jiang Lu, Da Zhang Dai, Xiao Lin Xu*, Bin Wei, Shi Yang Sun

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The strain D4 was identified as Enterobacter sp. according to its morphological characteristics and 16S rDNA sequence. Based on the single factor experiments, biodegradation conditions of the strain D4 were modeled and optimized by Plackett-Burman design (PB) and response surface method. The rotation speed, dibenzothiophene (DBT) concentration and reaction temperature were then selected as significant degradation factors. The optimal conditions for degradation were determined as follows: 175 r/min of rotation speed, 0.1 mmol/L of dibenzothiophene concentration and 30°C of culture temperature. Under the optimum conditions, the degradation rate was 59.83%, approximately 10% higher than that under original conditions.

Original languageEnglish
Pages (from-to)106-110
Number of pages5
JournalBeijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume32
Issue number1
Publication statusPublished - Jan 2012

Keywords

  • Biodegradation
  • Dibenzoth-iophene
  • Enterobacter sp. D4
  • Plackett-Burman design
  • Response surface method

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Guo, Z. G., Lu, J. J., Dai, D. Z., Xu, X. L., Wei, B., & Sun, S. Y. (2012). Optimization of biodegradation of dibenzothiophene using response surface methodology. Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, 32(1), 106-110.