One-step synthesis of PY-NBD to distinguish Cys/Hcy and GSH in aqueous solutions and living cells by dual channels

Meng Xu Gao; Sa Su; Chun Lei Yang; Hao Kang; Chen Lu Liang; Jing Jing; Xiao Ling Zhang

doi:10.1039/d1nj06165h

One-step synthesis of PY-NBD to distinguish Cys/Hcy and GSH in aqueous solutions and living cells by dual channels

Meng Xu Gao, Sa Su, Chun Lei Yang, Hao Kang, Chen Lu Liang, Jing Jing^*, Xiao Ling Zhang^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Common biothiols including Cys/Hcy and GSH are critical to the occurrence of both physiological and pathological processes; thus, the selective identification of Cys, Hcy and GSH is essential. In this study, by anchoring 7-nitrobenzo-2-oxa-1,3-diazole (NBD) with pyrene in a single step, an easy-to-synthesize probe PY-NBD was obtained. It showed dual-channel emission in response towards the biothiols, Cys/Hcy in blue and green and GSH in blue. In addition, in HeLa cells, PY-NBD was also effectively employed in the identification and differentiation of Cys/Hcy and GSH.

Original language	English
Pages (from-to)	6715-6719
Number of pages	5
Journal	New Journal of Chemistry
Volume	46
Issue number	14
DOIs	https://doi.org/10.1039/d1nj06165h
Publication status	Published - 28 Feb 2022

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title = "One-step synthesis of PY-NBD to distinguish Cys/Hcy and GSH in aqueous solutions and living cells by dual channels",

abstract = "Common biothiols including Cys/Hcy and GSH are critical to the occurrence of both physiological and pathological processes; thus, the selective identification of Cys, Hcy and GSH is essential. In this study, by anchoring 7-nitrobenzo-2-oxa-1,3-diazole (NBD) with pyrene in a single step, an easy-to-synthesize probe PY-NBD was obtained. It showed dual-channel emission in response towards the biothiols, Cys/Hcy in blue and green and GSH in blue. In addition, in HeLa cells, PY-NBD was also effectively employed in the identification and differentiation of Cys/Hcy and GSH.",

author = "Gao, {Meng Xu} and Sa Su and Yang, {Chun Lei} and Hao Kang and Liang, {Chen Lu} and Jing Jing and Zhang, {Xiao Ling}",

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T1 - One-step synthesis of PY-NBD to distinguish Cys/Hcy and GSH in aqueous solutions and living cells by dual channels

AU - Gao, Meng Xu

AU - Su, Sa

AU - Yang, Chun Lei

AU - Kang, Hao

AU - Liang, Chen Lu

AU - Jing, Jing

AU - Zhang, Xiao Ling

PY - 2022/2/28

Y1 - 2022/2/28

N2 - Common biothiols including Cys/Hcy and GSH are critical to the occurrence of both physiological and pathological processes; thus, the selective identification of Cys, Hcy and GSH is essential. In this study, by anchoring 7-nitrobenzo-2-oxa-1,3-diazole (NBD) with pyrene in a single step, an easy-to-synthesize probe PY-NBD was obtained. It showed dual-channel emission in response towards the biothiols, Cys/Hcy in blue and green and GSH in blue. In addition, in HeLa cells, PY-NBD was also effectively employed in the identification and differentiation of Cys/Hcy and GSH.

AB - Common biothiols including Cys/Hcy and GSH are critical to the occurrence of both physiological and pathological processes; thus, the selective identification of Cys, Hcy and GSH is essential. In this study, by anchoring 7-nitrobenzo-2-oxa-1,3-diazole (NBD) with pyrene in a single step, an easy-to-synthesize probe PY-NBD was obtained. It showed dual-channel emission in response towards the biothiols, Cys/Hcy in blue and green and GSH in blue. In addition, in HeLa cells, PY-NBD was also effectively employed in the identification and differentiation of Cys/Hcy and GSH.

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U2 - 10.1039/d1nj06165h

DO - 10.1039/d1nj06165h

M3 - Article

AN - SCOPUS:85128156359

SN - 1144-0546

VL - 46

SP - 6715

EP - 6719

JO - New Journal of Chemistry

JF - New Journal of Chemistry

IS - 14

ER -

One-step synthesis of PY-NBD to distinguish Cys/Hcy and GSH in aqueous solutions and living cells by dual channels

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