Molecularly imprinted hollow sphere array for the sensing of proteins

Wei Chen; Min Xue; Kenneth J. Shea; Zihui Meng; Zequn Yan; Zhe Wang; Fei Xue; Feng Qu

doi:10.1002/jbio.201400100

Molecularly imprinted hollow sphere array for the sensing of proteins

Wei Chen, Min Xue^*, Kenneth J. Shea, Zihui Meng, Zequn Yan, Zhe Wang, Fei Xue, Feng Qu

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Mono-dispersed molecularly imprinted hollow spheres (MIHSs) for hemoglobin (Hb) were prepared by employing silica nanospheres as the sacrificial templates. The obtained hollow spheres with uniform particle size of 360 nm in diameter were characterized by transmission electron microscopy. The outstanding affinities of these MIHSs to the target protein were confirmed by adsorption experiment in aqueous solution. Adsorption equilibrium was achieved within 10 min while the binding capacity (Q_max) of Hb was 8.84 μmol g^-1 at pH7.0. Furthermore, the MIHSs were successfully assembled into a closely-packed 3D colloidal array. The molecularly imprinted hollow sphere array (MIHSA) can selectively recognize Hb. As the concentration of Hb increased, the structure color of the MIHSA changed from blue to green, and turn to white finally with maximum red shift for 43 nm. The MIHSA showed promising potential for the naked-eye detection of target Hb.

Original language	English
Pages (from-to)	838-845
Number of pages	8
Journal	Journal of Biophotonics
Volume	8
Issue number	10
DOIs	https://doi.org/10.1002/jbio.201400100
Publication status	Published - Oct 2015

Keywords

Biosensor
Hemoglobin
Molecularly imprinted polymer
Naked-eye detection
Photonic crystal

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10.1002/jbio.201400100

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title = "Molecularly imprinted hollow sphere array for the sensing of proteins",

abstract = "Mono-dispersed molecularly imprinted hollow spheres (MIHSs) for hemoglobin (Hb) were prepared by employing silica nanospheres as the sacrificial templates. The obtained hollow spheres with uniform particle size of 360 nm in diameter were characterized by transmission electron microscopy. The outstanding affinities of these MIHSs to the target protein were confirmed by adsorption experiment in aqueous solution. Adsorption equilibrium was achieved within 10 min while the binding capacity (Qmax) of Hb was 8.84 μmol g-1 at pH7.0. Furthermore, the MIHSs were successfully assembled into a closely-packed 3D colloidal array. The molecularly imprinted hollow sphere array (MIHSA) can selectively recognize Hb. As the concentration of Hb increased, the structure color of the MIHSA changed from blue to green, and turn to white finally with maximum red shift for 43 nm. The MIHSA showed promising potential for the naked-eye detection of target Hb.",

keywords = "Biosensor, Hemoglobin, Molecularly imprinted polymer, Naked-eye detection, Photonic crystal",

author = "Wei Chen and Min Xue and Shea, {Kenneth J.} and Zihui Meng and Zequn Yan and Zhe Wang and Fei Xue and Feng Qu",

note = "Publisher Copyright: {\textcopyright} 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2015",

month = oct,

doi = "10.1002/jbio.201400100",

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T1 - Molecularly imprinted hollow sphere array for the sensing of proteins

AU - Chen, Wei

AU - Xue, Min

AU - Shea, Kenneth J.

AU - Meng, Zihui

AU - Yan, Zequn

AU - Wang, Zhe

AU - Xue, Fei

AU - Qu, Feng

PY - 2015/10

Y1 - 2015/10

N2 - Mono-dispersed molecularly imprinted hollow spheres (MIHSs) for hemoglobin (Hb) were prepared by employing silica nanospheres as the sacrificial templates. The obtained hollow spheres with uniform particle size of 360 nm in diameter were characterized by transmission electron microscopy. The outstanding affinities of these MIHSs to the target protein were confirmed by adsorption experiment in aqueous solution. Adsorption equilibrium was achieved within 10 min while the binding capacity (Qmax) of Hb was 8.84 μmol g-1 at pH7.0. Furthermore, the MIHSs were successfully assembled into a closely-packed 3D colloidal array. The molecularly imprinted hollow sphere array (MIHSA) can selectively recognize Hb. As the concentration of Hb increased, the structure color of the MIHSA changed from blue to green, and turn to white finally with maximum red shift for 43 nm. The MIHSA showed promising potential for the naked-eye detection of target Hb.

AB - Mono-dispersed molecularly imprinted hollow spheres (MIHSs) for hemoglobin (Hb) were prepared by employing silica nanospheres as the sacrificial templates. The obtained hollow spheres with uniform particle size of 360 nm in diameter were characterized by transmission electron microscopy. The outstanding affinities of these MIHSs to the target protein were confirmed by adsorption experiment in aqueous solution. Adsorption equilibrium was achieved within 10 min while the binding capacity (Qmax) of Hb was 8.84 μmol g-1 at pH7.0. Furthermore, the MIHSs were successfully assembled into a closely-packed 3D colloidal array. The molecularly imprinted hollow sphere array (MIHSA) can selectively recognize Hb. As the concentration of Hb increased, the structure color of the MIHSA changed from blue to green, and turn to white finally with maximum red shift for 43 nm. The MIHSA showed promising potential for the naked-eye detection of target Hb.

KW - Biosensor

KW - Hemoglobin

KW - Molecularly imprinted polymer

KW - Naked-eye detection

KW - Photonic crystal

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SP - 838

EP - 845

JO - Journal of Biophotonics

JF - Journal of Biophotonics

IS - 10

ER -

Molecularly imprinted hollow sphere array for the sensing of proteins

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Keywords

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