TY - JOUR
T1 - Mixta tenebrionis sp. Nov., isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.
AU - Xia, Mengli
AU - Wang, Jialei
AU - Huo, Yi Xin
AU - Yang, Yu
N1 - Publisher Copyright:
© 2020 The Authors.
PY - 2020
Y1 - 2020
N2 - A bacterial strain, BIT-26T, was isolated from the gut of plastic-eating mealworm Tenebrio molitor L. The taxonomic position of this new isolate was investigated by using a polyphasic approach. Cells of the strain were Gram-stain-negative, facultatively anaerobic, motile rods with peritrichous flagella. The 16S rRNA gene sequence (1412 bp) of strain BIT-26T showed the highest similarity (97.4%) to Erwinia piriflorinigrans CFBP 5888T, followed by Citrobacter sedlakii NBRC 105722T (97.3%), Mixta calida LMG 25383T (97.3%), Cronobacter muytjensii ATCC 51329T (97.2%) and Mixta theicola QC88-366T (97.2%). The results of phylogenetic analyses, based on the 16S rRNA gene and concatenated sequences of four housekeeping genes (atpD, gyrB, infB and rpoB), placed strain BIT-26T within the genus Mixta of the family Erwiniaceae. This affiliation was also supported by the chemotaxonomic data. Strain BIT-26T had similar predominant fatty acids, including C12: 0, C14: 0, C16: 0, C17: 0 cyclo and C19: 0 cyclo ω8c, to species of the genus Mixta. In silico DNA–DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain BIT-26T from other species of the genus Mixta with validly published names. Therefore, strain BIT-26T is considered to represent a novel species, for which the name Mixta tenebrionis sp. nov is proposed. The type strain is BIT-26T (=CGMCC 1.17041T=KCTC 72449T).
AB - A bacterial strain, BIT-26T, was isolated from the gut of plastic-eating mealworm Tenebrio molitor L. The taxonomic position of this new isolate was investigated by using a polyphasic approach. Cells of the strain were Gram-stain-negative, facultatively anaerobic, motile rods with peritrichous flagella. The 16S rRNA gene sequence (1412 bp) of strain BIT-26T showed the highest similarity (97.4%) to Erwinia piriflorinigrans CFBP 5888T, followed by Citrobacter sedlakii NBRC 105722T (97.3%), Mixta calida LMG 25383T (97.3%), Cronobacter muytjensii ATCC 51329T (97.2%) and Mixta theicola QC88-366T (97.2%). The results of phylogenetic analyses, based on the 16S rRNA gene and concatenated sequences of four housekeeping genes (atpD, gyrB, infB and rpoB), placed strain BIT-26T within the genus Mixta of the family Erwiniaceae. This affiliation was also supported by the chemotaxonomic data. Strain BIT-26T had similar predominant fatty acids, including C12: 0, C14: 0, C16: 0, C17: 0 cyclo and C19: 0 cyclo ω8c, to species of the genus Mixta. In silico DNA–DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain BIT-26T from other species of the genus Mixta with validly published names. Therefore, strain BIT-26T is considered to represent a novel species, for which the name Mixta tenebrionis sp. nov is proposed. The type strain is BIT-26T (=CGMCC 1.17041T=KCTC 72449T).
KW - Gut bacteria
KW - Mealworm
KW - Mixta
KW - Plastic biodegradation
KW - Polystyrene
UR - http://www.scopus.com/inward/record.url?scp=85081118074&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.003826
DO - 10.1099/ijsem.0.003826
M3 - Article
C2 - 31675291
AN - SCOPUS:85081118074
SN - 1466-5026
VL - 70
SP - 790
EP - 796
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 2
M1 - 003826
ER -