Fu, R., Su, Y., Wang, R., Lin, X., Jiang, K., Jin, X., Yang, H., Ma, L., Luo, X., Lu, Y., & Huang, G. (2019). Label-free tomography of living cellular nanoarchitecture using hyperspectral selfinterference microscopy. Biomedical Optics Express, 10(6), 2757-2767. https://doi.org/10.1364/BOE.10.002757
Fu, Rongxin ; Su, Ya ; Wang, Ruliang et al. / Label-free tomography of living cellular nanoarchitecture using hyperspectral selfinterference microscopy. In: Biomedical Optics Express. 2019 ; Vol. 10, No. 6. pp. 2757-2767.
@article{4c68824fd76749aab3917c4944496c6f,
title = "Label-free tomography of living cellular nanoarchitecture using hyperspectral selfinterference microscopy",
abstract = "Quantitative phase imaging (QPI) is the most ideal method for achieving long-term cellular tomography because it is label free and quantitative. However, for current QPI instruments, interference signals from different layers overlay with each other and impede nanoscale optical sectioning. Integrated incubators and improved configurations also require further investigation for QPI instruments. In this work, hyperspectral self-reflectance microscopy is proposed to achieve label-free tomography of living cellular nanoarchitecture. The optical description and tomography reconstruction algorithm were proposed so that the quantitative morphological structure of the entire living cell can be acquired with 89.2 nm axial resolution and 1.91 nm optical path difference sensitivity. A cell incubator was integrated to culture living cells for in situ measurement and expensive precise optical components were not needed. The proposed system can reveal native and dynamic cellular nanoscale structure, providing an alternative approach for long-term monitoring and quantitative analysis of living cells.",
author = "Rongxin Fu and Ya Su and Ruliang Wang and Xue Lin and Kai Jiang and Xiangyu Jin and Han Yang and Li Ma and Xianbo Luo and Ying Lu and Guoliang Huang",
note = "Publisher Copyright: {\textcopyright} 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.",
year = "2019",
month = jun,
day = "1",
doi = "10.1364/BOE.10.002757",
language = "English",
volume = "10",
pages = "2757--2767",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "Optica Publishing Group (formerly OSA)",
number = "6",
}
Fu, R, Su, Y, Wang, R, Lin, X, Jiang, K, Jin, X, Yang, H, Ma, L, Luo, X, Lu, Y & Huang, G 2019, 'Label-free tomography of living cellular nanoarchitecture using hyperspectral selfinterference microscopy', Biomedical Optics Express, vol. 10, no. 6, pp. 2757-2767. https://doi.org/10.1364/BOE.10.002757
Label-free tomography of living cellular nanoarchitecture using hyperspectral selfinterference microscopy. /
Fu, Rongxin; Su, Ya; Wang, Ruliang et al.
In:
Biomedical Optics Express, Vol. 10, No. 6, 01.06.2019, p. 2757-2767.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Label-free tomography of living cellular nanoarchitecture using hyperspectral selfinterference microscopy
AU - Fu, Rongxin
AU - Su, Ya
AU - Wang, Ruliang
AU - Lin, Xue
AU - Jiang, Kai
AU - Jin, Xiangyu
AU - Yang, Han
AU - Ma, Li
AU - Luo, Xianbo
AU - Lu, Ying
AU - Huang, Guoliang
N1 - Publisher Copyright:
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Quantitative phase imaging (QPI) is the most ideal method for achieving long-term cellular tomography because it is label free and quantitative. However, for current QPI instruments, interference signals from different layers overlay with each other and impede nanoscale optical sectioning. Integrated incubators and improved configurations also require further investigation for QPI instruments. In this work, hyperspectral self-reflectance microscopy is proposed to achieve label-free tomography of living cellular nanoarchitecture. The optical description and tomography reconstruction algorithm were proposed so that the quantitative morphological structure of the entire living cell can be acquired with 89.2 nm axial resolution and 1.91 nm optical path difference sensitivity. A cell incubator was integrated to culture living cells for in situ measurement and expensive precise optical components were not needed. The proposed system can reveal native and dynamic cellular nanoscale structure, providing an alternative approach for long-term monitoring and quantitative analysis of living cells.
AB - Quantitative phase imaging (QPI) is the most ideal method for achieving long-term cellular tomography because it is label free and quantitative. However, for current QPI instruments, interference signals from different layers overlay with each other and impede nanoscale optical sectioning. Integrated incubators and improved configurations also require further investigation for QPI instruments. In this work, hyperspectral self-reflectance microscopy is proposed to achieve label-free tomography of living cellular nanoarchitecture. The optical description and tomography reconstruction algorithm were proposed so that the quantitative morphological structure of the entire living cell can be acquired with 89.2 nm axial resolution and 1.91 nm optical path difference sensitivity. A cell incubator was integrated to culture living cells for in situ measurement and expensive precise optical components were not needed. The proposed system can reveal native and dynamic cellular nanoscale structure, providing an alternative approach for long-term monitoring and quantitative analysis of living cells.
UR - http://www.scopus.com/inward/record.url?scp=85067955157&partnerID=8YFLogxK
U2 - 10.1364/BOE.10.002757
DO - 10.1364/BOE.10.002757
M3 - Article
AN - SCOPUS:85067955157
SN - 2156-7085
VL - 10
SP - 2757
EP - 2767
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 6
ER -
Fu R, Su Y, Wang R, Lin X, Jiang K, Jin X et al. Label-free tomography of living cellular nanoarchitecture using hyperspectral selfinterference microscopy. Biomedical Optics Express. 2019 Jun 1;10(6):2757-2767. doi: 10.1364/BOE.10.002757