Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level

Ren Li; Mingxing Zhou; Chunyan Yue; Weikai Zhang; Zewen Wei; Zhiyuan Hu

Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level

Ren Li, Mingxing Zhou, Chunyan Yue, Weikai Zhang, Zewen Wei^*, Zhiyuan Hu

^*Corresponding author for this work

National Center for Nanoscience and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

By integrating single cell trapping, identifying and in situ MDA (Multiple Displacement Amplification) gene amplifying in a single microchip, here we report a simple and cost-effective method to not only detect the existence of EGFR (Epidermal Growth Factor Receptor) mutation at single cell level, but also determine the abundances of diverse cells exhibiting different EGFR mutation types which were clinically proved to significantly affect the prognosis of EGFR-targeted therapy.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	369-370
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Externally published	Yes
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016

Publication series

Name	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	9/10/16 → 13/10/16

Keywords

Epidermal growth factor receptor mutation
Microfluidic
Single cell analysis

Cite this

Li, R., Zhou, M., Yue, C., Zhang, W., Wei, Z., & Hu, Z. (2016). Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 369-370). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.

Li, Ren ; Zhou, Mingxing ; Yue, Chunyan et al. / Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 369-370 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

@inproceedings{ff265ee3cf1748f3aa4c6feed0e5ac66,

title = "Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level",

abstract = "By integrating single cell trapping, identifying and in situ MDA (Multiple Displacement Amplification) gene amplifying in a single microchip, here we report a simple and cost-effective method to not only detect the existence of EGFR (Epidermal Growth Factor Receptor) mutation at single cell level, but also determine the abundances of diverse cells exhibiting different EGFR mutation types which were clinically proved to significantly affect the prognosis of EGFR-targeted therapy.",

keywords = "Epidermal growth factor receptor mutation, Microfluidic, Single cell analysis",

author = "Ren Li and Mingxing Zhou and Chunyan Yue and Weikai Zhang and Zewen Wei and Zhiyuan Hu",

year = "2016",

language = "English",

series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society",

pages = "369--370",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

}

Li, R, Zhou, M, Yue, C, Zhang, W, Wei, Z & Hu, Z 2016, Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 369-370, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 9/10/16.

Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level. / Li, Ren; Zhou, Mingxing; Yue, Chunyan et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 369-370 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level

AU - Li, Ren

AU - Zhou, Mingxing

AU - Yue, Chunyan

AU - Zhang, Weikai

AU - Wei, Zewen

AU - Hu, Zhiyuan

PY - 2016

Y1 - 2016

N2 - By integrating single cell trapping, identifying and in situ MDA (Multiple Displacement Amplification) gene amplifying in a single microchip, here we report a simple and cost-effective method to not only detect the existence of EGFR (Epidermal Growth Factor Receptor) mutation at single cell level, but also determine the abundances of diverse cells exhibiting different EGFR mutation types which were clinically proved to significantly affect the prognosis of EGFR-targeted therapy.

AB - By integrating single cell trapping, identifying and in situ MDA (Multiple Displacement Amplification) gene amplifying in a single microchip, here we report a simple and cost-effective method to not only detect the existence of EGFR (Epidermal Growth Factor Receptor) mutation at single cell level, but also determine the abundances of diverse cells exhibiting different EGFR mutation types which were clinically proved to significantly affect the prognosis of EGFR-targeted therapy.

KW - Epidermal growth factor receptor mutation

KW - Microfluidic

KW - Single cell analysis

UR - http://www.scopus.com/inward/record.url?scp=85014135703&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85014135703

T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

SP - 369

EP - 370

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Y2 - 9 October 2016 through 13 October 2016

ER -

Li R, Zhou M, Yue C, Zhang W, Wei Z, Hu Z. Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 369-370. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Identifying EGFR-expressed cells and determining the abundances of different EGFR mutation types at single-cell level

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Keywords

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