Highly photostable two-photon NIR AIEgens with tunable organelle specificity and deep tissue penetration

Guangle Niu; Ruoyao Zhang; Yuan Gu; Jianguo Wang; Chao Ma; Ryan T.K. Kwok; Jacky W.Y. Lam; Herman H.Y. Sung; Ian D. Williams; Kam Sing Wong; Xiaoqiang Yu; Ben Zhong Tang

doi:10.1016/j.biomaterials.2019.04.002

Highly photostable two-photon NIR AIEgens with tunable organelle specificity and deep tissue penetration

Guangle Niu, Ruoyao Zhang, Yuan Gu, Jianguo Wang, Chao Ma, Ryan T.K. Kwok, Jacky W.Y. Lam, Herman H.Y. Sung, Ian D. Williams, Kam Sing Wong, Xiaoqiang Yu, Ben Zhong Tang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

Abstract

Photostability is a particularly important parameter for fluorescence imaging especially long-term dynamic tracking in live samples. However, many organic fluorophores show poor photostability under one-photon and two-photon continuous irradiation. In addition, these traditional fluorophores also suffer from aggregation-caused quenching (ACQ) in aggregate state in insolvable water environment. Therefore, it remains challenging to develop photostable and ACQ-free fluorophores for biological imaging. In this work, we developed two highly photostable aggregation-induced emission luminogens (AIEgens) based on the cyanostilbene core for in vitro and ex vivo bioimaging. These AIEgens named CS-Py ⁺ SO ₃ ⁻ and CS-Py ⁺ exhibit near-infrared solid-state emission, large Stokes shift (>180 nm), high fluorescence quantum yield (12.8%–13.7%) and good two-photon absorption cross section (up to 88 GM). CS-Py ⁺ SO ₃ ⁻ and CS-Py ⁺ show specific organelle staining with high biocompatibility in membrane and mitochondria in live cells, respectively. In addition, selective two-photon mitochondria visualization in live rat skeletal muscle tissues with deep-tissue penetration (about 100 μm) is successfully realized by using CS-Py ⁺ . Furthermore, these AIEgens especially CS-Py ⁺ exhibit remarkably high resistance to photobleaching under one-photon and two-photon continuous irradiation. These highly photostable AIEgens could be potentially utilized in visualizing and tracking specific organelle-associated dynamic changes in live systems.

Original language	English
Pages (from-to)	72-82
Number of pages	11
Journal	Biomaterials
Volume	208
DOIs	https://doi.org/10.1016/j.biomaterials.2019.04.002
Publication status	Published - Jul 2019
Externally published	Yes

Keywords

AIEgen
Deep tissue penetration
High photostability
Near-infarared
Two-photon

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10.1016/j.biomaterials.2019.04.002

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@article{9b518de6427e4f4abad1d7becf531bbd,

title = "Highly photostable two-photon NIR AIEgens with tunable organelle specificity and deep tissue penetration",

abstract = " Photostability is a particularly important parameter for fluorescence imaging especially long-term dynamic tracking in live samples. However, many organic fluorophores show poor photostability under one-photon and two-photon continuous irradiation. In addition, these traditional fluorophores also suffer from aggregation-caused quenching (ACQ) in aggregate state in insolvable water environment. Therefore, it remains challenging to develop photostable and ACQ-free fluorophores for biological imaging. In this work, we developed two highly photostable aggregation-induced emission luminogens (AIEgens) based on the cyanostilbene core for in vitro and ex vivo bioimaging. These AIEgens named CS-Py + SO 3 − and CS-Py + exhibit near-infrared solid-state emission, large Stokes shift (>180 nm), high fluorescence quantum yield (12.8%–13.7%) and good two-photon absorption cross section (up to 88 GM). CS-Py + SO 3 − and CS-Py + show specific organelle staining with high biocompatibility in membrane and mitochondria in live cells, respectively. In addition, selective two-photon mitochondria visualization in live rat skeletal muscle tissues with deep-tissue penetration (about 100 μm) is successfully realized by using CS-Py + . Furthermore, these AIEgens especially CS-Py + exhibit remarkably high resistance to photobleaching under one-photon and two-photon continuous irradiation. These highly photostable AIEgens could be potentially utilized in visualizing and tracking specific organelle-associated dynamic changes in live systems.",

keywords = "AIEgen, Deep tissue penetration, High photostability, Near-infarared, Two-photon",

author = "Guangle Niu and Ruoyao Zhang and Yuan Gu and Jianguo Wang and Chao Ma and Kwok, {Ryan T.K.} and Lam, {Jacky W.Y.} and Sung, {Herman H.Y.} and Williams, {Ian D.} and Wong, {Kam Sing} and Xiaoqiang Yu and Tang, {Ben Zhong}",

note = "Publisher Copyright: {\textcopyright} 2019",

year = "2019",

month = jul,

doi = "10.1016/j.biomaterials.2019.04.002",

language = "English",

volume = "208",

pages = "72--82",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Highly photostable two-photon NIR AIEgens with tunable organelle specificity and deep tissue penetration

AU - Niu, Guangle

AU - Zhang, Ruoyao

AU - Gu, Yuan

AU - Wang, Jianguo

AU - Ma, Chao

AU - Kwok, Ryan T.K.

AU - Lam, Jacky W.Y.

AU - Sung, Herman H.Y.

AU - Williams, Ian D.

AU - Wong, Kam Sing

AU - Yu, Xiaoqiang

AU - Tang, Ben Zhong

PY - 2019/7

Y1 - 2019/7

N2 - Photostability is a particularly important parameter for fluorescence imaging especially long-term dynamic tracking in live samples. However, many organic fluorophores show poor photostability under one-photon and two-photon continuous irradiation. In addition, these traditional fluorophores also suffer from aggregation-caused quenching (ACQ) in aggregate state in insolvable water environment. Therefore, it remains challenging to develop photostable and ACQ-free fluorophores for biological imaging. In this work, we developed two highly photostable aggregation-induced emission luminogens (AIEgens) based on the cyanostilbene core for in vitro and ex vivo bioimaging. These AIEgens named CS-Py + SO 3 − and CS-Py + exhibit near-infrared solid-state emission, large Stokes shift (>180 nm), high fluorescence quantum yield (12.8%–13.7%) and good two-photon absorption cross section (up to 88 GM). CS-Py + SO 3 − and CS-Py + show specific organelle staining with high biocompatibility in membrane and mitochondria in live cells, respectively. In addition, selective two-photon mitochondria visualization in live rat skeletal muscle tissues with deep-tissue penetration (about 100 μm) is successfully realized by using CS-Py + . Furthermore, these AIEgens especially CS-Py + exhibit remarkably high resistance to photobleaching under one-photon and two-photon continuous irradiation. These highly photostable AIEgens could be potentially utilized in visualizing and tracking specific organelle-associated dynamic changes in live systems.

AB - Photostability is a particularly important parameter for fluorescence imaging especially long-term dynamic tracking in live samples. However, many organic fluorophores show poor photostability under one-photon and two-photon continuous irradiation. In addition, these traditional fluorophores also suffer from aggregation-caused quenching (ACQ) in aggregate state in insolvable water environment. Therefore, it remains challenging to develop photostable and ACQ-free fluorophores for biological imaging. In this work, we developed two highly photostable aggregation-induced emission luminogens (AIEgens) based on the cyanostilbene core for in vitro and ex vivo bioimaging. These AIEgens named CS-Py + SO 3 − and CS-Py + exhibit near-infrared solid-state emission, large Stokes shift (>180 nm), high fluorescence quantum yield (12.8%–13.7%) and good two-photon absorption cross section (up to 88 GM). CS-Py + SO 3 − and CS-Py + show specific organelle staining with high biocompatibility in membrane and mitochondria in live cells, respectively. In addition, selective two-photon mitochondria visualization in live rat skeletal muscle tissues with deep-tissue penetration (about 100 μm) is successfully realized by using CS-Py + . Furthermore, these AIEgens especially CS-Py + exhibit remarkably high resistance to photobleaching under one-photon and two-photon continuous irradiation. These highly photostable AIEgens could be potentially utilized in visualizing and tracking specific organelle-associated dynamic changes in live systems.

KW - AIEgen

KW - Deep tissue penetration

KW - High photostability

KW - Near-infarared

KW - Two-photon

UR - http://www.scopus.com/inward/record.url?scp=85064514725&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2019.04.002

DO - 10.1016/j.biomaterials.2019.04.002

M3 - Article

C2 - 30999153

AN - SCOPUS:85064514725

SN - 0142-9612

VL - 208

SP - 72

EP - 82

JO - Biomaterials

JF - Biomaterials

ER -

Highly photostable two-photon NIR AIEgens with tunable organelle specificity and deep tissue penetration

Abstract

Keywords

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