Glycogen synthase kinase-3β facilitates cell apoptosis induced by high fluence low-power laser irradiation through acceleration of Bax translocation

Lei Huang, Shengnan Wu, Da Xing*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Glycogen synthase kinase-3β (GSK-3β) is a critical activator of cell apoptosis induced by a diverse array of insults. However, the effects of GSK-3β on the human lung adenocarcinoma cell (ASTC-a-1) apoptosis induced by high fluence low-power laser irradiation (HF-LPLI) are not clear. Here, we showed that GSK-3β was constantly translocated from cytoplasm to nucleus and activated during HF-LPLI-induced cell apoptosis. In addition, we found that co-overexpression of YFP-GSK-3β and CFP-Bax in ASTC-a-1 cells accelerated both Bax translocations to mitochondria and cell apoptosis, compared to the cells expressed CFP-Bax only under HF-LPLI treatment, indicating that GSK-3β facilitated ASTC-a-1 cells apoptosis through acceleration mitochondrial translocation of Bax. Our results demonstrate that GSK-3β exerts some of its pro-apoptotic effects in ASTC-a-1 cells by regulating the mitochondrial localization of Bax, a key component of the intrinsic apoptotic cascade.

Original languageEnglish
Title of host publicationMechanisms for Low-Light Therapy VI
DOIs
Publication statusPublished - 2011
Externally publishedYes
EventMechanisms for Low-Light Therapy VI - San Francisco, CA, United States
Duration: 22 Jan 201123 Jan 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7887
ISSN (Print)1605-7422

Conference

ConferenceMechanisms for Low-Light Therapy VI
Country/TerritoryUnited States
CitySan Francisco, CA
Period22/01/1123/01/11

Keywords

  • Apoptosis
  • Bax
  • GSK-3β
  • HF-LPLI

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