Huang, G., Huang, Q., Ma, L., Luo, X., Pang, B., Zhang, Z., Wang, R., Zhang, J., Li, Q., Fu, R., & Ye, J. (2014). FM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor. Scientific Reports, 4, Article 7344. https://doi.org/10.1038/srep07344
Huang, Guoliang ; Huang, Qin ; Ma, Li et al. / FM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor. In: Scientific Reports. 2014 ; Vol. 4.
@article{3ea5c6a4cf48439288562794ada5be84,
title = "FM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor",
abstract = "A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit. The non-stick-coated metal microfluidic bioreactor, with a surface contact angle of 103°, was largely inert to bio-molecules, and DNA amplification could be performed in a minimum reaction volume of 40 nL. The isothermal nucleic acid amplification for Mycoplasma pneumoniae identification in the non-stick-coated microfluidic bioreactor could be performed at a minimum DNA template concentration of 1.3 aM, and a detection limit of three copies of genomic DNA was obtained. This microfluidic bioreactor offers a promising clinically relevant pathogen molecular diagnostic method via the amplification of targets from only a few copies of genomic DNA from a single bacterium.",
author = "Guoliang Huang and Qin Huang and Li Ma and Xianbo Luo and Biao Pang and Zhixin Zhang and Ruliang Wang and Junqi Zhang and Qi Li and Rongxin Fu and Jiancheng Ye",
year = "2014",
doi = "10.1038/srep07344",
language = "English",
volume = "4",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}
Huang, G, Huang, Q, Ma, L, Luo, X, Pang, B, Zhang, Z, Wang, R, Zhang, J, Li, Q, Fu, R & Ye, J 2014, 'FM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor', Scientific Reports, vol. 4, 7344. https://doi.org/10.1038/srep07344
FM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor. / Huang, Guoliang; Huang, Qin; Ma, Li et al.
In:
Scientific Reports, Vol. 4, 7344, 2014.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - FM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor
AU - Huang, Guoliang
AU - Huang, Qin
AU - Ma, Li
AU - Luo, Xianbo
AU - Pang, Biao
AU - Zhang, Zhixin
AU - Wang, Ruliang
AU - Zhang, Junqi
AU - Li, Qi
AU - Fu, Rongxin
AU - Ye, Jiancheng
PY - 2014
Y1 - 2014
N2 - A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit. The non-stick-coated metal microfluidic bioreactor, with a surface contact angle of 103°, was largely inert to bio-molecules, and DNA amplification could be performed in a minimum reaction volume of 40 nL. The isothermal nucleic acid amplification for Mycoplasma pneumoniae identification in the non-stick-coated microfluidic bioreactor could be performed at a minimum DNA template concentration of 1.3 aM, and a detection limit of three copies of genomic DNA was obtained. This microfluidic bioreactor offers a promising clinically relevant pathogen molecular diagnostic method via the amplification of targets from only a few copies of genomic DNA from a single bacterium.
AB - A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoliter reaction assays in real-time, with fluorescence collection near the optical diffraction limit. The non-stick-coated metal microfluidic bioreactor, with a surface contact angle of 103°, was largely inert to bio-molecules, and DNA amplification could be performed in a minimum reaction volume of 40 nL. The isothermal nucleic acid amplification for Mycoplasma pneumoniae identification in the non-stick-coated microfluidic bioreactor could be performed at a minimum DNA template concentration of 1.3 aM, and a detection limit of three copies of genomic DNA was obtained. This microfluidic bioreactor offers a promising clinically relevant pathogen molecular diagnostic method via the amplification of targets from only a few copies of genomic DNA from a single bacterium.
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U2 - 10.1038/srep07344
DO - 10.1038/srep07344
M3 - Article
C2 - 25475544
AN - SCOPUS:84923370192
SN - 2045-2322
VL - 4
JO - Scientific Reports
JF - Scientific Reports
M1 - 7344
ER -
Huang G, Huang Q, Ma L, Luo X, Pang B, Zhang Z et al. FM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor. Scientific Reports. 2014;4:7344. doi: 10.1038/srep07344