Feasibility Study of Label-Free Raman Spectroscopy for Parathyroid Gland Identification

Hao Wang, Sisi Guo, Ruoyu Zhang*, Jing Yao, Wen Tian*, Jianfeng Wang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We aim to evaluate the feasibility of Raman spectroscopy for parathyroid gland (PG) identification during thyroidectomy. Using a novel side-viewing handheld Raman probe, a total of 324 Raman spectra of four tissue types (i.e., thyroid, lymph node, PG, and lipid) commonly encountered during thyroidectomy were rapidly (< 3 s) acquired from 80 tissue sites (thyroid [n = 10], lymph node [n = 10], PG [n = 40], lipid [n = 20]) of 10 euthanized Wistar rats. Two partial least-squares (PLS)-discriminant analysis (DA) detection models were developed, differentiating the lipid and nonlipid (i.e., thyroid, lymph node, and PG) tissues with an accuracy of 100%, and PG, lymph node, and thyroid could be detected with an accuracy of 98.4%, 93.9%, and 95.4% respectively. This work demonstrates the feasibility of Raman spectroscopy technique for PG identification and protection during thyroidectomy at the molecular level.

Original languageEnglish
Article numbere202400220
JournalJournal of Biophotonics
Volume18
Issue number1
DOIs
Publication statusPublished - Jan 2025

Keywords

  • Raman spectroscopy
  • parathyroid gland identification
  • partial least-squares-discriminant analysis
  • thyroidectomy

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Wang, H., Guo, S., Zhang, R., Yao, J., Tian, W., & Wang, J. (2025). Feasibility Study of Label-Free Raman Spectroscopy for Parathyroid Gland Identification. Journal of Biophotonics, 18(1), Article e202400220. https://doi.org/10.1002/jbio.202400220