TY - GEN
T1 - Fast neutron irradiation induces regionally specific activation of TRAIL/DR5 pathway in rat brain
AU - Rao, Ling
AU - Wang, Hailong
AU - Shan, Zhengang
AU - Zheng, Jieying
AU - Wang, Xiao
AU - Ma, Hong
PY - 2013
Y1 - 2013
N2 - Object To explore the effects of neutron-irradiation exposure on TRAIL/DRS pathway in rat brain regions. Methods Fast neutron-irradiation was employed as the irradiated model. The expression changes of TRAIL and DRS in cerebral cortex, hippocampus, cerebellum and corpus striatum were detected after 1, 7 and 14 d post-irradiation by RT-PCR, ELISA and western blotting. Cleaved caspase-8 was examined by western blotting. Results In protein expression level, TRAIL was increased in the four regions to different extents after 7 d and then recovered after 14 d; DRS was detected to be up-regulated in cerebral cortex, hippocampus and corpus striatum but down-regulated in cerebellum after 14 d post-irradiation. No significant changes of TRAIL and DRS were observed in RNA level by RT-PCR. The cleaved caspase-8 was up-expressed the greatest after 1 d in cerebral cortex, hippocampus and cerebellum but continually up-regulated in corpus striatum from 1 to 14 d after irradiation treatment. Conclusion TRAIL was induced in rat brain at early stage while the expression changes of DRS were delayed by neutron-irradiation and vary in different brain regions. Moreover, caspase-8 was not activated in accord with the changes of TRAIL and DRS in some brain regions. These indicate that the activation of TRAIL/DRS pathway plays a role in irradiation-induced injury, which is regulated in a region-specific and time-dependent manner.
AB - Object To explore the effects of neutron-irradiation exposure on TRAIL/DRS pathway in rat brain regions. Methods Fast neutron-irradiation was employed as the irradiated model. The expression changes of TRAIL and DRS in cerebral cortex, hippocampus, cerebellum and corpus striatum were detected after 1, 7 and 14 d post-irradiation by RT-PCR, ELISA and western blotting. Cleaved caspase-8 was examined by western blotting. Results In protein expression level, TRAIL was increased in the four regions to different extents after 7 d and then recovered after 14 d; DRS was detected to be up-regulated in cerebral cortex, hippocampus and corpus striatum but down-regulated in cerebellum after 14 d post-irradiation. No significant changes of TRAIL and DRS were observed in RNA level by RT-PCR. The cleaved caspase-8 was up-expressed the greatest after 1 d in cerebral cortex, hippocampus and cerebellum but continually up-regulated in corpus striatum from 1 to 14 d after irradiation treatment. Conclusion TRAIL was induced in rat brain at early stage while the expression changes of DRS were delayed by neutron-irradiation and vary in different brain regions. Moreover, caspase-8 was not activated in accord with the changes of TRAIL and DRS in some brain regions. These indicate that the activation of TRAIL/DRS pathway plays a role in irradiation-induced injury, which is regulated in a region-specific and time-dependent manner.
KW - Fast neutron-irradiation
KW - Rat brain regions
KW - TRAIL/DR5 pathway
UR - http://www.scopus.com/inward/record.url?scp=84881498064&partnerID=8YFLogxK
U2 - 10.1109/ICCME.2013.6548242
DO - 10.1109/ICCME.2013.6548242
M3 - Conference contribution
AN - SCOPUS:84881498064
SN - 9781467329699
T3 - 2013 ICME International Conference on Complex Medical Engineering, CME 2013
SP - 215
EP - 218
BT - 2013 ICME International Conference on Complex Medical Engineering, CME 2013
T2 - 2013 7th ICME International Conference on Complex Medical Engineering, CME 2013
Y2 - 25 May 2013 through 28 May 2013
ER -