Fast and parallel detection of Four Ebola Virus species on a microfluidic-chip-based portable reverse transcription loop-mediated isothermal amplification system

Xue Lin, Xiangyu Jin, Bin Xu, Ruliang Wang, Rongxin Fu, Ya Su, Kai Jiang, Han Yang, Ying Lu, Yong Guo, Guoliang Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Considering the lack of official vaccines and medicines for Ebola virus infection, reliable diagnostic methods are necessary for the control of the outbreak and the spread of the disease. We developed a microfluidic-chip-based portable system for fast and parallel detection of four Ebola virus species. The system is based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and consists of four specific LAMP primers, a disc microfluidic chip, and a portable real-time fluorescence detector. It could specifically and parallelly distinguish four species of the Ebola virus after only one sampling, including the Zaire Ebola virus, the Sudan Ebola virus, the Bundibugyo Ebola virus, and the Tai Forest Ebola virus, without cross-contamination. The limit of detection was as small as 10 copies per reaction, while the total consumption of sample and reagent was 0.94 μL per reaction. The final results could be obtained in 50 min after one addition of sample and reagent mixture. This approach provides simplicity, high sensitivity, and multi-target parallel detection at a low cost, which could enable convenient and effective on-site detections of the Ebola virus in the outdoors, remote areas, and modern hospitals.

Original languageEnglish
Article number777
JournalMicromachines
Volume10
Issue number11
DOIs
Publication statusPublished - 1 Nov 2019
Externally publishedYes

Keywords

  • Detection
  • Ebola virus
  • Microfluidic chip
  • Reverse transcription loop-mediated isothermal amplification

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Lin, X., Jin, X., Xu, B., Wang, R., Fu, R., Su, Y., Jiang, K., Yang, H., Lu, Y., Guo, Y., & Huang, G. (2019). Fast and parallel detection of Four Ebola Virus species on a microfluidic-chip-based portable reverse transcription loop-mediated isothermal amplification system. Micromachines, 10(11), Article 777. https://doi.org/10.3390/mi10110777