TY - GEN
T1 - Fabrication and assembly of multi-layered microstructures embedding cells inside microfluidic devices
AU - Yue, Tao
AU - Nakajima, Masahiro
AU - Wang, Huaping
AU - Hu, Chengzhi
AU - Takeuchi, Masaru
AU - Fukuda, Toshio
PY - 2013
Y1 - 2013
N2 - Recently the research about constructing 3 dimensional cell structures is very important for its great potential applications in tissue engineering. In this paper, we report a novel method of constructing multi-layered microstructures embedding cells via microfluidic devices. The on-chip fabrication of movable microstructures embedding fibroblasts (NIH/3T3) based on Poly (ethylene glycol) Diacrylate (PEGDA) was reported. Two approaches for assembling these movable microstructures were presented. One was a manual assembly method based on micromanipulation system and the other one was a self-assembly method based on microfluidic channel. Several manual assembly ways were demonstrated and a tube-shaped microstructure with 17 layers was assembled by an efficient assembly method. A novel microfluidic channel was presented for conducting self-assembly method and a 2-layered experimental microfluidic device was fabricated by Polydimethylsiloxane (PDMS). The self-assembly process of fabricated microstructures via this device was preliminarily demonstrated.
AB - Recently the research about constructing 3 dimensional cell structures is very important for its great potential applications in tissue engineering. In this paper, we report a novel method of constructing multi-layered microstructures embedding cells via microfluidic devices. The on-chip fabrication of movable microstructures embedding fibroblasts (NIH/3T3) based on Poly (ethylene glycol) Diacrylate (PEGDA) was reported. Two approaches for assembling these movable microstructures were presented. One was a manual assembly method based on micromanipulation system and the other one was a self-assembly method based on microfluidic channel. Several manual assembly ways were demonstrated and a tube-shaped microstructure with 17 layers was assembled by an efficient assembly method. A novel microfluidic channel was presented for conducting self-assembly method and a 2-layered experimental microfluidic device was fabricated by Polydimethylsiloxane (PDMS). The self-assembly process of fabricated microstructures via this device was preliminarily demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=84893786969&partnerID=8YFLogxK
U2 - 10.1109/IROS.2013.6696400
DO - 10.1109/IROS.2013.6696400
M3 - Conference contribution
AN - SCOPUS:84893786969
SN - 9781467363587
T3 - IEEE International Conference on Intelligent Robots and Systems
SP - 514
EP - 519
BT - IROS 2013
T2 - 2013 26th IEEE/RSJ International Conference on Intelligent Robots and Systems: New Horizon, IROS 2013
Y2 - 3 November 2013 through 8 November 2013
ER -