TY - JOUR
T1 - Extraction of shikimic acid from chinese star anise using flash column chromatography on a molecularly-imprinted polymer column
AU - Xue, Min
AU - Wang, Yu
AU - Meng, Zihui
AU - Zhang, Weibing
AU - Wu, Yin
AU - Jiang, Shikuan
PY - 2013/11/26
Y1 - 2013/11/26
N2 - Flash column chromatography prepacked with molecularly imprinted polymers (MIPs) has been successfully used for the extraction of shikimic acid from Chinese star anise. The shikimic acid imprinted polymer with an adsorption capacity of 21.94 μmol g-1 dry polymer and imprinting factor of 4.2 was prepared using the dispersion polymerization method. The capacity of the MIP-Flash column was determined as 23.3 mg g-1 using a frontal chromatography method when 10 m mol L-1 shikimic acid was eluted through the MIP-flash column at 1.7 mL min-1. The crude extract of Chinese star anise loaded on the column was eluted with ethanol/water (40/60, v/v) to remove the impurities, after elution with methanol/water/acetic acid (32/48/20, v/v/v), purified shikimic acid (>95%) was obtained. The MIP-flash column can be reused for at least 4 rounds with capacity lost less than 15%. It was demonstrated that, compared with HPLC, flash column chromatography prepacked with MIPs is a more rapid, feasible, and economical method for the extraction of active ingredients from plants on a large scale based on the technology of molecular imprinting.
AB - Flash column chromatography prepacked with molecularly imprinted polymers (MIPs) has been successfully used for the extraction of shikimic acid from Chinese star anise. The shikimic acid imprinted polymer with an adsorption capacity of 21.94 μmol g-1 dry polymer and imprinting factor of 4.2 was prepared using the dispersion polymerization method. The capacity of the MIP-Flash column was determined as 23.3 mg g-1 using a frontal chromatography method when 10 m mol L-1 shikimic acid was eluted through the MIP-flash column at 1.7 mL min-1. The crude extract of Chinese star anise loaded on the column was eluted with ethanol/water (40/60, v/v) to remove the impurities, after elution with methanol/water/acetic acid (32/48/20, v/v/v), purified shikimic acid (>95%) was obtained. The MIP-flash column can be reused for at least 4 rounds with capacity lost less than 15%. It was demonstrated that, compared with HPLC, flash column chromatography prepacked with MIPs is a more rapid, feasible, and economical method for the extraction of active ingredients from plants on a large scale based on the technology of molecular imprinting.
KW - HPLC
KW - molecularly imprinted technology
KW - natural product
KW - polymer chemistry
KW - proteomics
KW - separation science and technology
UR - http://www.scopus.com/inward/record.url?scp=84882411885&partnerID=8YFLogxK
U2 - 10.1080/10826076.2012.725690
DO - 10.1080/10826076.2012.725690
M3 - Article
AN - SCOPUS:84882411885
SN - 1082-6076
VL - 36
SP - 2677
EP - 2686
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 19
ER -