Endoplasmic Reticulum-Directed Ratiometric Fluorescent Probe for Quantitive Detection of Basal H2O2

Congcong Gao; Yong Tian; Rubo Zhang; Jing Jing; Xiaoling Zhang

doi:10.1021/acs.analchem.7b03809

Endoplasmic Reticulum-Directed Ratiometric Fluorescent Probe for Quantitive Detection of Basal H₂O₂

Congcong Gao, Yong Tian, Rubo Zhang^*, Jing Jing, Xiaoling Zhang

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

120 Citations (Scopus)

Abstract

The endoplasmic reticulum (ER) has a central role in the fine-tuning of environmental and internal stimuli. We herein report a ratiometric fluorescent probe, α-Naph, capable of determining basal H₂O₂ in the ER. The probe specifically responds to H₂O₂. The limit of detection of the probe is as low as 38 nM, making it a feasible sensor to image intracellular basal H₂O₂. In addition, utilizing its ratiometric property, we are able to measure the concentration of H₂O₂ in the ER quantitatively, eliminating the error caused by the probe concentration and environment. The intracellular concentration of H₂O₂ in the ER is calculated to be 0.692 μM under normal conditions and 1.26 μM under the stimulation of phorbol myristate acetate.

Original language	English
Pages (from-to)	12945-12950
Number of pages	6
Journal	Analytical Chemistry
Volume	89
Issue number	23
DOIs	https://doi.org/10.1021/acs.analchem.7b03809
Publication status	Published - 5 Dec 2017

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title = "Endoplasmic Reticulum-Directed Ratiometric Fluorescent Probe for Quantitive Detection of Basal H2O2",

abstract = "The endoplasmic reticulum (ER) has a central role in the fine-tuning of environmental and internal stimuli. We herein report a ratiometric fluorescent probe, α-Naph, capable of determining basal H2O2 in the ER. The probe specifically responds to H2O2. The limit of detection of the probe is as low as 38 nM, making it a feasible sensor to image intracellular basal H2O2. In addition, utilizing its ratiometric property, we are able to measure the concentration of H2O2 in the ER quantitatively, eliminating the error caused by the probe concentration and environment. The intracellular concentration of H2O2 in the ER is calculated to be 0.692 μM under normal conditions and 1.26 μM under the stimulation of phorbol myristate acetate.",

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AU - Gao, Congcong

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AU - Zhang, Xiaoling

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AB - The endoplasmic reticulum (ER) has a central role in the fine-tuning of environmental and internal stimuli. We herein report a ratiometric fluorescent probe, α-Naph, capable of determining basal H2O2 in the ER. The probe specifically responds to H2O2. The limit of detection of the probe is as low as 38 nM, making it a feasible sensor to image intracellular basal H2O2. In addition, utilizing its ratiometric property, we are able to measure the concentration of H2O2 in the ER quantitatively, eliminating the error caused by the probe concentration and environment. The intracellular concentration of H2O2 in the ER is calculated to be 0.692 μM under normal conditions and 1.26 μM under the stimulation of phorbol myristate acetate.

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Endoplasmic Reticulum-Directed Ratiometric Fluorescent Probe for Quantitive Detection of Basal H₂O₂

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