TY - JOUR
T1 - Detection of organophosphorus compounds using a molecularly imprinted photonic crystal
AU - Liu, Feng
AU - Huang, Shuyue
AU - Xue, Fei
AU - Wang, Yifei
AU - Meng, Zihui
AU - Xue, Min
PY - 2012/2/15
Y1 - 2012/2/15
N2 - A label free molecularly imprinted photonic crystal (MIPC) was developed to detect the degradation product of nerve agents. Mono-dispersed poly-methyl methacrylate colloidal particles with the diameter of 280nm were used to fabricate a closely packed colloidal crystal array (CCA), and a methyl phosphonic acid (MPA) imprinted hydrogel was prepared within the CCA using 2-hydroxyethyl-methacrylate and N-isopropylacrylamide as monomers, ethyleneglycol dimethacrylate and N, N'-methylenebisacrylamide as cross-linkers, a mixture of n-octanol and acetonitrile as porogen. The diffraction intensity of the MIPC decreased significantly upon the MPA adsorption with a limit of detection (LOD) of 10 -6molL -1. Furthermore, the diffraction intensity decreased and blue shifted with the increase of temperature, decreased and red shifted with the increase of ionic strength. At higher pH, the diffraction intensity increased without obvious diffraction shift. The MIPC provides an indirect path to detect nerve agents (Sarin, Soman, VX and R-VX) by monitoring the MPA released from the hydrolysis of nerve agents, with LODs of 3.5×10 -6molL -1, 2.5×10 -5molL -1, 7.5×10 -5molL -1 and 7.5×10 -5molL -1 for Sarin, Soman, VX and R-VX, respectively.
AB - A label free molecularly imprinted photonic crystal (MIPC) was developed to detect the degradation product of nerve agents. Mono-dispersed poly-methyl methacrylate colloidal particles with the diameter of 280nm were used to fabricate a closely packed colloidal crystal array (CCA), and a methyl phosphonic acid (MPA) imprinted hydrogel was prepared within the CCA using 2-hydroxyethyl-methacrylate and N-isopropylacrylamide as monomers, ethyleneglycol dimethacrylate and N, N'-methylenebisacrylamide as cross-linkers, a mixture of n-octanol and acetonitrile as porogen. The diffraction intensity of the MIPC decreased significantly upon the MPA adsorption with a limit of detection (LOD) of 10 -6molL -1. Furthermore, the diffraction intensity decreased and blue shifted with the increase of temperature, decreased and red shifted with the increase of ionic strength. At higher pH, the diffraction intensity increased without obvious diffraction shift. The MIPC provides an indirect path to detect nerve agents (Sarin, Soman, VX and R-VX) by monitoring the MPA released from the hydrolysis of nerve agents, with LODs of 3.5×10 -6molL -1, 2.5×10 -5molL -1, 7.5×10 -5molL -1 and 7.5×10 -5molL -1 for Sarin, Soman, VX and R-VX, respectively.
KW - Molecular imprinting
KW - Nerve agents
KW - Organophosphonic compounds
KW - Photonic crystal
UR - http://www.scopus.com/inward/record.url?scp=84855820458&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2011.11.012
DO - 10.1016/j.bios.2011.11.012
M3 - Article
C2 - 22196876
AN - SCOPUS:84855820458
SN - 0956-5663
VL - 32
SP - 273
EP - 277
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -