Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA

Jingge Wang, Chaoyong Huang, Kai Guo, Lianjie Ma, Xiangyu Meng, Ning Wang*, Yi Xin Huo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Escherichia coli strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production. However, the direct application of this strain suffers from the transformation inefficiency and plasmid instability. Herein, we conducted genetic modifications at a serial of loci of MG1655 genome, generating a robust and universal host strain JW128 with higher transformation efficiency and plasmid stability that can be used to efficiently produce desired chemicals after introducing the corresponding synthetic pathways. Using JW128 as the host, the titer of isobutanol reached 5.76 g/L in shake-flask fermentation, and the titer of lycopene reached 1.91 g/L in test-tube fermentation, 40-fold and 5-fold higher than that of original MG1655, respectively. These results demonstrated JW128 is a promising chassis for high-level production of value-added chemicals.

Original languageEnglish
Pages (from-to)333-342
Number of pages10
JournalSynthetic and Systems Biotechnology
Volume5
Issue number4
DOIs
Publication statusPublished - Dec 2020

Keywords

  • CRISPR‐Cas9
  • Defense system
  • Escherichia coli
  • MG1655
  • Metabolic engineering

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