@inproceedings{d5ed972a42c849d0a33a35705bfb067f,
title = "Construction and validation of a crispr/dcpf1-based transcriptional regulatory system in saccharomyces cerevisiae",
abstract = "Saccharomyces cerevisiae has been extensively studied and applied as a model microbial platform for efficient and sustainable production of biofuels, chemicals and natural products. In recent years, the application of CRISPR system in genome editing and transcriptional regulation has greatly improved the efficiency of microbial genome editing. To achieve transcriptional regulation the CRISPR/dCpf1-mediated CRISPRa/i transcriptional regulation system was constructed in this study. By constructing crRNA arrays and adding different activation proteins, repression efficiencies from 62% to 177% for the CRISPRi system and activation efficiencies from 154% to 320% for the CRISPRa system were achieved in the eGFP gene reporter system.",
keywords = "CRISPR-dCpf1, CRISPRa/i, Saccharomyces cerevisiae",
author = "Qing Feng and Jun Li",
note = "Publisher Copyright: {\textcopyright} 2023 IOS Press. All rights reserved.; 3rd International Conference on Biomedicine and Bioinformatics Engineering, ICBBE 2023 ; Conference date: 16-06-2023 Through 18-06-2023",
year = "2023",
month = nov,
day = "23",
doi = "10.3233/SHTI230900",
language = "English",
series = "Studies in Health Technology and Informatics",
publisher = "IOS Press BV",
pages = "680--688",
editor = "Yitao Yu and Nguyen, {Binh P.} and Jun Sang",
booktitle = "Advances in Biomedical and Bioinformatics Engineering - Proceedings of the 3rd International Conference on Biomedicine and Bioinformatics Engineering, ICBBE 2023",
address = "Netherlands",
}