A “Turn-on” fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein

Xiaoling Pan; Pai Liu; Xinghui Wu; Yahui Zhang; Zhengxu Cai; Jianbing Shi; Junge Zhi; Zi Li; Dayan Wang; Bin Tong; Yuping Dong

doi:10.1016/j.snb.2021.130392

A “Turn-on” fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein

Xiaoling Pan, Pai Liu, Xinghui Wu, Yahui Zhang, Zhengxu Cai, Jianbing Shi, Junge Zhi, Zi Li, Dayan Wang, Bin Tong^*, Yuping Dong

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.

Original language	English
Article number	130392
Journal	Sensors and Actuators, B: Chemical
Volume	345
DOIs	https://doi.org/10.1016/j.snb.2021.130392
Publication status	Published - 15 Oct 2021

Keywords

Aggregation-induced emission
Hemagglutinin
Hexaphenyl-1,3-butadiene derivatives
“Turn-on” fluorescent response

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10.1016/j.snb.2021.130392

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Pan, X., Liu, P., Wu, X., Zhang, Y., Cai, Z., Shi, J., Zhi, J., Li, Z., Wang, D., Tong, B., & Dong, Y. (2021). A “Turn-on” fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein. Sensors and Actuators, B: Chemical, 345, Article 130392. https://doi.org/10.1016/j.snb.2021.130392

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abstract = "Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.",

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AU - Pan, Xiaoling

AU - Liu, Pai

AU - Wu, Xinghui

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AU - Cai, Zhengxu

AU - Shi, Jianbing

AU - Zhi, Junge

AU - Li, Zi

AU - Wang, Dayan

AU - Tong, Bin

AU - Dong, Yuping

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AB - Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.

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A “Turn-on” fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein

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