A “Turn-on” fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein

Xiaoling Pan; Pai Liu; Xinghui Wu; Yahui Zhang; Zhengxu Cai; Jianbing Shi; Junge Zhi; Zi Li; Dayan Wang; Bin Tong; Yuping Dong

doi:10.1016/j.snb.2021.130392

A “Turn-on” fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein

Xiaoling Pan, Pai Liu, Xinghui Wu, Yahui Zhang, Zhengxu Cai, Jianbing Shi, Junge Zhi, Zi Li, Dayan Wang, Bin Tong^*, Yuping Dong

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.

Original language	English
Article number	130392
Journal	Sensors and Actuators, B: Chemical
Volume	345
DOIs	https://doi.org/10.1016/j.snb.2021.130392
Publication status	Published - 15 Oct 2021

Keywords

Aggregation-induced emission
Hemagglutinin
Hexaphenyl-1,3-butadiene derivatives
“Turn-on” fluorescent response

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10.1016/j.snb.2021.130392

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title = "A “Turn-on” fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein",

abstract = "Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.",

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author = "Xiaoling Pan and Pai Liu and Xinghui Wu and Yahui Zhang and Zhengxu Cai and Jianbing Shi and Junge Zhi and Zi Li and Dayan Wang and Bin Tong and Yuping Dong",

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doi = "10.1016/j.snb.2021.130392",

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AU - Pan, Xiaoling

AU - Liu, Pai

AU - Wu, Xinghui

AU - Zhang, Yahui

AU - Cai, Zhengxu

AU - Shi, Jianbing

AU - Zhi, Junge

AU - Li, Zi

AU - Wang, Dayan

AU - Tong, Bin

AU - Dong, Yuping

PY - 2021/10/15

Y1 - 2021/10/15

N2 - Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.

AB - Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.

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KW - Hemagglutinin

KW - Hexaphenyl-1,3-butadiene derivatives

KW - “Turn-on” fluorescent response

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A “Turn-on” fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein

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Keywords

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