A new stable and reliable method for labeling nucleic acids of fully replicative viruses

Li Li Huang, Peng Zhou, Han Zhong Wang, Rui Zhang, Jian Hao, Hai Yan Xie*, Zhi Ke He

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Efficiently labeling nucleic acids of fully replicative viruses is a challenge. In this work, a ‘molecular light switch’ complex [Ru(phen)2(dppz)]2+, where phen = 1,10-phenanthroline and dppz = dipyrido[3,2-a:2′,3′-c]phenazine, has been exploringly used to label vaccinia virus nucleic acid. The labeled virions exhibited strong and stable fluorescence and could be imaged at the single-virion level. Moreover, they were fully infectious and can be used to study the behaviors of invasion into their host cells. The method is general and suitable for labeling various DNA viruses.

Original languageEnglish
Pages (from-to)2424-2426
Number of pages3
JournalChemical Communications
Volume48
Issue number18
DOIs
Publication statusPublished - 1 Feb 2012

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Huang, L. L., Zhou, P., Wang, H. Z., Zhang, R., Hao, J., Xie, H. Y., & He, Z. K. (2012). A new stable and reliable method for labeling nucleic acids of fully replicative viruses. Chemical Communications, 48(18), 2424-2426. https://doi.org/10.1039/c2cc17069h